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https://repositorio.inpa.gov.br/handle/1/37398
Title: | Production of antimicrobial metabolites against pathogenic bacteria and yeasts by Fusarium oxysporum in submerged culture processes |
Authors: | Poleto, Liliane da Rosa, Letícia Osório Fontana, Roselei Claudete Rodrigues, Eliseu Poletto, Édina Baldo, Guilherme Paesi, Suelen Osmarina Campos, Ceci Sales da Gama Camassola, Marli |
Keywords: | Antibiotics Biopharmaceuticals Antimicrobial agents Antimicrobial resistance Screening test |
Issue Date: | 2021 |
metadata.dc.publisher.journal: | Bioprocess and Biosystems Engineering |
Abstract: | The antimicrobial activity of the metabolites produced by Fusarium oxysporum PR-33 in submerged culture was evaluated against Gram-positive and Gram-negative bacteria and yeasts. Metabolites were determined by HPLC–DAD-MS/MS. An extract was obtained following the removal of mycelium by centrifugation and lyophilisation of the supernatant. The compounds in this extract demonstrated broad-spectrum antimicrobial action, with rates of inhibition between 60 and 80%, depending on the species and extract tested. The major compounds of the extracts were identified as fusarinolic acid and its isomer [56.9% flask extract (FE)] and 59.2% bioreactor extract (BE), dehydrofusaric acid (35.7% FE and 31.6% BE), and fusaric acid (6.5% FE and 1.1% BE). Fusaric acid has been shown to be responsible for antimicrobial activity. The cytotoxicity of the extracts was evaluated in culture of HEK-293 and SH-SY5Y animal cells and toxicity of these extracts was verified even in the lowest tested concentrations. Therefore, our results indicate that the compounds identified exhibit potential as antimicrobial agents. |
metadata.dc.identifier.doi: | https://doi.org/10.1007/s00449-021-02538-2 |
Appears in Collections: | Artigos |
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