Mapeamento Cromossômico e Expressão Gênica do Elemento Retrotransponível Rex1 em Colossoma macropomum (Serrasalmidae, Characiformes) submetidos ao metal pesado Cobre (Cu++)

Abstract

The transposable elements are known for their ability to move and integrate into the genome ofthe host organism randomly. They have two classifications: Class I is the retrotransposons' class that have as an intermediary transposition RNA, which from it is synthesized cDNA and this is inserted into the genome. The retrotransposons are classified in two ways, those with long terminal repeats, called retrotransposons LTRs and those who do not have this repetition is called retrotransposons not - LTRs. The retrotransposons not - LTRs can still be classified as LINEs (long interspersed elements) and SINES (short interspersed elements); Class II is composed of DNA transposons, which have their own DNA as an intermediary migrating or being directly copied and inserted into the genome. The Rex1 is retrotransposon not-LTR of Rex family and is found in several types of organisms. Several studies indicate that the Rex family retrotransposons have the capacity to respond to environmental stress. In this study we were made chromosome mapping and the absolute quantification Rex1 in Colossoma macropomum that have undergone environmental stress by water contamination with copper. The results corroborate the hypothesis that this retrotransposon has a response to that kind of stress in the muscle tissue of the Tambaqui. Found in chromosome mapping by fluorescence in situ hybridization, highest number oftags in animals which were subjected to stress and images were quantified in the FLIMA software, confirming that data through the highest fluorescence ratio. In Tambaqui absolute quantitation of muscle samples from the PCR Real - Time also found larger numbers ofcopies ofRex1 the samples were subjected to environmental stress copper.