Diversidade e identificação molecular de isolados de colletotrichum associados ao gênero capsicum no Amazonas

Abstract

Colletotrichum species of the genus are widely distributed in temperate, tropical and subtrotropicais regions and cause a disease known as anthracnose in numerous genres of host plants. It is common to find several Colletotrichum species pathogenic to the same host, as it is a species common cause disease symptoms in various host plants. The cultivation of chillies and peppers are often attacked by several species of Colletotrichum and registers high losses in economic output due to damage caused by the disease. Thus, the correct identification of the causative agent is essential for defining control strategies and integrated management, so that the development of an effective management strategy depends, among other factors, the understanding of the etiology of disease, genetic diversity and structure the population of the pathogen. In order to identify the species of Colletotrichum responsible for causing anthracnose in the Capsicum plant fruits in the State of Amazonas, this study analyzed 94 isolates from fruits pepper, pepper and fragrant murupi pepper. The identification was based on multilocus sequencing and analysis of partial regions of the ACT gene, CHS, GAPDH, HIS and GS and allowed the identification of seven different species of Colletotrichum associated with anthracnose lesions in fruits of Capsicum plants (C. scovillei C. truncatum, C. brevisporum, C. siamense, C. fruticola, C. theobromicola and C. gloeosporioides). It was possible to confirm the predominance of C. scovillei in the municipalities of Presidente Figueiredo and Rio Preto da Eva, C. siamense in the city of Manacapuru, C. brevisporum in Manacapuru and C. truncatum exclusively in the municipality of Iranduba. The pathogenic species of Colletotrichum are widespread in the State of Amazonas affecting the health of plantations and causing economic losses to producers, confirming the cosmopolitan distribution, low pathogenic specificity and high evolutionary potential of the species belonging to this genre.