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Title: Cytogenetics, genomics and biodiversity of the South American and African Arapaimidae fish family (Teleostei, Osteoglossiformes)
Authors: Oliveira, Ezequiel Aguiar de
Bertollo, Luiz Antônio Carlos
Ráb, Petr
Ezaz, Tariq Tariq
Yano, Cassia Fernanda
Hatanaka, Terumi
Jegede, Oladele Ilesanmi
Tanomtong, Alongklod
Liehr, Thomas
Sember, Alexandr
Maruyama, Sandra R.
Feldberg, Eliana
Viana, Patrik Ferreira
Cioffi, Marcelo de Bello
Keywords: Dna 18s
Dna 5s
Repetitive Dna
Ribosome Dna
Acrocentric Chromosome
Animals Cell
Animals Tissue
Arapaima Gigas
C Banding
Chromosomal Mapping
Chromosome Analysis
Chromosome Number
Comparative Genomic Hybridization
Controlled Study
Dna Content
In Situ Hybridization, Fluorescence
Genetic Distance
Genetic Variability
Heterotis Niloticus
Principal Component Analysis
Polymorphism, Single Nucleotide
South America
Polymorphism, Single Nucleotide
South Africa
Ribosomal Dna
Polymorphism, Single Nucleotide
South Africa
Issue Date: 2019
metadata.dc.publisher.journal: PLoS ONE
metadata.dc.relation.ispartof: Volume 14, Número 3
Abstract: Osteoglossiformes represents one of the most ancestral teleost lineages, currently widespread over almost all continents, except for Antarctica. However, data involving advanced molecular cytogenetics or comparative genomics are yet largely limited for this fish group. Therefore, the present investigations focus on the osteoglossiform family Arapaimidae, studying a unique fish model group with advanced molecular cytogenetic genomic tools. The aim is to better explore and clarify certain events and factors that had impact on evolutionary history of this fish group. For that, both South American and African representatives of Arapaimidae, namely Arapaima gigas and Heterotis niloticus, were examined. Both species differed markedly by diploid chromosome numbers, with 2n = 56 found in A. gigas and 2n = 40 exhibited by H. niloticus. Conventional cytogenetics along with fluorescence in situ hybridization revealed some general trends shared by most osteoglossiform species analyzed thus far, such as the presence of only one chromosome pair bearing 18S and 5S rDNA sites and karyotypes dominated by acrocentric chromosomes, resembling thus the patterns of hypothetical ancestral teleost karyotype. Furthermore, the genomes of A. gigas and H. niloticus display remarkable divergence in terms of repetitive DNA content and distribution, as revealed by comparative genomic hybridization (CGH). On the other hand, genomic diversity of single copy sequences studied through principal component analyses (PCA) based on SNP alleles genotyped by the DArT seq procedure demonstrated a very low genetic distance between the South American and African Arapaimidae species; this pattern contrasts sharply with the scenario found in other osteoglossiform species. Underlying evolutionary mechanisms potentially explaining the obtained data have been suggested and discussed. © 2019 de Oliveira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
metadata.dc.identifier.doi: 10.1371/journal.pone.0214225
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