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Title: Protein analysis and gene expression indicate differential vulnerability of Iberian fish species under a climate change scenario
Authors: Jesus, Tiago Filipe
Moreno, João M.
Repolho, T.
Athanasiadis, Alekos
Rosa, Rui
Val, Vera Maria Fonseca Almeida e
Coelho, Maria Manuela
Keywords: Fk 506 Binding Protein
Fk 506 Binding Protein 4
Fk 506 Binding Protein 52
Guanine Nucleotide Binding Protein
Heat Shock Cognate Protein 70
Heat Shock Protein
Heat Shock Protein 90
Hypoxia Inducible Factor 1alpha
Protein Gpb1
Protein Hsp90aa1
Protein Stip1
Unclassified Drug
Fish Protein
Animals Experiment
Animals Tissue
Circadian Rhythm
Climate Change
Controlled Study
Coping Behavior
Cry1aa Gene
Cs Gene
Endangered Species
Energy Metabolism
Energy Yield
Environmental Change
Freshwater Fish
Gbp1 Gene
Gene Expression
Gene Function
Immune Response
Ldh A Gene
Nucleotide Sequence
Per1a Gene
Protein Analysis
Protein Folding
Protein Stability
Protein Structure
Species Comparison
Species Conservations
Squalius Carolitertii
Squalius Torgalensis
Endangered Species
Gene Expression Regulation
Evolution, Molecular
Molecular Model
Protein Conformation
Climate Change
Endangered Species
Evolution, Molecular
Fish Proteins
Gene Expression Regulation
Models, Molecular
Protein Conformation
Issue Date: 2017
metadata.dc.publisher.journal: PLoS ONE
metadata.dc.relation.ispartof: Volume 12, Número 7
Abstract: Current knowledge on the biological responses of freshwater fish under projected scenarios of climate change remains limited. Here, we examine differences in the protein configuration of two endemic Iberian freshwater fish species, Squalius carolitertii and the critically endangered S. torgalensis that inhabit in the Atlantic-type northern and in the Mediterranean-type southwestern regions, respectively. We performed protein structure modeling of fourteen genes linked to protein folding, energy metabolism, circadian rhythms and immune responses. Structural differences in proteins between the two species were found for HSC70, FKBP52, HIF1α and GPB1. For S. torgalensis, besides structural differences, we found higher thermostability for two proteins (HSP90 and GBP1), which can be advantageous in a warmer environment. Additionally, we investigated how these species might respond to projected scenarios of 3 climate change warming, acidification (ΔpH = -0.4), and their combined effects. Significant changes in gene expression were observed in response to all treatments, particularly under the combined warming and acidification. While S. carolitertii presented changes in gene expression for multiple proteins related to folding (hsp90aa1, hsc70, fkbp4 and stip1), only one such gene was altered in S. torgalensis (stip1). However, S. torgalensis showed a greater capacity for energy production under both the acidification and combined scenarios by increasing cs gene expression and maintaining ldha gene expression in muscle. Overall, these findings suggest that S. torgalensis is better prepared to cope with projected climate change. Worryingly, under the simulated scenarios, disturbances to circadian rhythm and immune system genes (cry1aa, per1a and gbp1) raise concerns for the persistence of both species, highlighting the need to consider multi-stressor effects when evaluating climate change impacts upon fish. This work also highlights that assessments of the potential of endangered freshwater species to cope with environmental change are crucial to help decision-makers adopt future conservation strategies. © 2017 Jesus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
metadata.dc.identifier.doi: 10.1371/journal.pone.0181325
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