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Title: Anopheles aquasalis infected by Plasmodium vivax displays unique gene expression profiles when compared to other malaria vectors and plasmodia
Authors: Bahia, Ana Cristina
Kubota, Marina S.
Tempone, Antônio Jorge
Pinheiro, Waléria Dasso
Tadei, Wanderli Pedro
Secundino, Nagilá Francinete Costa
Traub-Csekö, Yara Maria
Pimenta, Paulo Filemon Paolucci
Keywords: Actin
Amino Acid Sequence
Biological Model
Expressed Sequence Tag
Gene Expression Profiling
Gene Expression Regulation
Gene Library
Molecular Genetics
Plasmodium Vivax
Reverse Transcription Polymerase Chain Reaction
Sequence Homology
Species Difference
Amino Acid Sequence
Expressed Sequence Tags
Gene Expression Profiling
Gene Expression Regulation
Gene Library
Models, Genetic
Molecular Sequence Data
Plasmodium Vivax
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Species Specificity
Issue Date: 2010
metadata.dc.publisher.journal: PLoS ONE
metadata.dc.relation.ispartof: Volume 5, Número 3
Abstract: Malaria affects 300 million people worldwide every year and is endemic in 22 countries in the Americas where transmission occurs mainly in the Amazon Region. Most malaria cases in the Americas are caused by Plasmodium vivax, a parasite that is almost impossible to cultivate in vitro, and Anopheles aquasalis is an important malaria vector. Understanding the interactions between this vector and its parasite will provide important information for development of disease control strategies. To this end, we performed mRNA subtraction experiments using A. aquasalis 2 and 24 hours after feeding on blood and blood from malaria patients infected with P. vivax to identify changes in the mosquito vector gene induction that could be important during the initial steps of infection. A total of 2,138 clones of differentially expressed genes were sequenced and 496 high quality unique sequences were obtained. Annotation revealed 36% of sequences unrelated to genes in any database, suggesting that they were specific to A. aquasalis. A high number of sequences (59%) with no matches in any databases were found 24 h after infection. Genes related to embryogenesis were down-regulated in insects infected by P. vivax. Only a handful of genes related to immune responses were detected in our subtraction experiment. This apparent weak immune response of A. aquasalis to P. vivax infection could be related to the susceptibility of this vector to this important human malaria parasite. Analysis of some genes by real time PCR corroborated and expanded the subtraction results. Taken together, these data provide important new information about this poorly studied American malaria vector by revealing differences between the responses of A. aquasalis to P. vivax infection, in relation to better studied mosquito-Plasmodium pairs. These differences may be important for the development of malaria transmissionblocking strategies in the Americas. © 2010 Bahia et al.
metadata.dc.identifier.doi: 10.1371/journal.pone.0009795
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