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Title: Genomic resources for the conservation and management of the harpy eagle (Harpia harpyja, Falconiformes, Accipitridae)
Authors: Banhos, Aureo
Hrbek, Tomas
Gravena, Waleska
Sanaiotti, Tânia Margarete
Farias, Izeni P.
Keywords: Genomic Dna
Animals Tissue
Conservation Genetics
Controlled Study
Dna Degradation
Dna Extraction
Gene Amplification
Gene Locus
Polymorphism, Genetic
Genetic Variability
Microsatellite Marker
Nucleotide Sequence
Sex Determination
Species Conservations
Statistical Significance
Wild Species
Harpia Harpyja
Morphnus Guianensis
Issue Date: 2008
metadata.dc.publisher.journal: Genetics and Molecular Biology
metadata.dc.relation.ispartof: Volume 31, Número 1, Pags. 146-154
Abstract: We report the characterization and optimization of 45 heterologous microsatellite loci, and the development of a new set of molecular sex markers for the conservation and management of the Neotropical harpy eagle (Harpia harpyja L. 1758). Of the 45 microsatellites tested, 24 were polymorphic, six monomorphic, 10 uncharacterizable due to multiple bands and five did not amplify. The observed gene diversity of the analyzed sample of H. harpyja was low and similar to that of other threatened Falconiformes. While a high proportion of the microsatellite markers were highly variable, individuals of H. harpyja could be differentiated by a joint analysis of just three (p = 2.79 × 10-4) or four markers (p = 2.89 × 10-4). Paternity could be rejected with 95.23% and 97.83% probabilities using the same three and four markers, respectively. The sex determination markers easily and consistently differentiated males from females even with highly degraded DNA extracted from naturally shed feathers. The markers reported in this study potentially provide an excellent set of molecular tools for the conservation and management of wild and captive H. harpyja and they may also prove useful for the enigmatic Neotropical crested eagle (Morphnus guianensis Daudin 1800). Copyright by the Brazilian Society of Genetics.
metadata.dc.identifier.doi: 10.1590/S1415-47572008000100025
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