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Título: | Ras oncogene and Hypoxia-inducible factor-1 alpha (Hif-1α) expression in the Amazon fish Colossoma macropomum (Cuvier, 1818) exposed to benzo(a)pyrene |
Autor: | Silva, Grazyelle Sebrenski da Fé, Luciana Mara Lopes Silva, Maria de Nazaré Paula da Val, Vera Maria Fonseca Almeida e |
Palavras-chave: | Benzo(a)pyrene Corn Oil Hypoxia Inducible Factor 1alpha Animals Cell Animals Experiment Animals Tissue Cell Membrane Cell Vacuole Colossoma Macropomum Comet Assay Concentration (parameters) Controlled Study Cytoplasm Exposure Gene Expression Gene Overexpression Genotoxicity Hif 1alpha Gene Histopathology Liver Cell Nucleus Liver Parenchyma Membrane Rupture Nonhuman Oncogene Ras Quantitative Analysis Real-time Polymerase Chain Reaction |
Data do documento: | 2017 |
Revista: | Genetics and Molecular Biology |
É parte de: | Volume 40, Número 2, Pags. 491-501 |
Abstract: | Benzo[a]pyrene (B[a]P) is a petroleum derivative capable of inducing cancer in human and animals. In this work, under laboratory conditions, we analyzed the responses of Colossoma macropomum to B[a]P acute exposure through intraperitoneal injection of four different B[a]P concentrations (4, 8, 16 and 32 μmol/kg) or corn oil (control group). We analyzed expression of the ras oncogene and the Hypoxia-inducible factor-1 alpha (hif-1α) gene using quantitative real-time PCR. Additionally, liver histopathological changes and genotoxic effects were evaluated through the comet assay. Ras oncogene was overexpressed in fish exposed to 4, 8 of 16 μmol/kg B[a]P, showing 4.96, 7.10 and 6.78-fold increases, respectively. Overexpression also occurred in hif-1α in fish injected with 4 and 8 μmol/kg B[a]P, showing 8.82 and 4.64-fold increases, respectively. Histopathological damage in fish liver was classified as irreparable in fish exposed to 8, 16 and 32 μmol/kg μM B[a]P. The genotoxic damage increased in fish injected with 8 and 16 μmol/kg in comparison with the control group. Acute exposure of B[a]P was capable to interrupt the expression of ras oncogene and hif-1α, and increase DNA breaks due to tissue damage. © 2017, Sociedade Brasileira de Genética. |
DOI: | 10.1590/1678-4685-GMB-2016-0066 |
Aparece nas coleções: | Artigos |
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