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Title: Effect of isodillapiole on the expression of the insecticide resistance genes GSTE7 and CYP6N12 in Aedes aegypti from central Amazonia
Authors: Lima, V. S.
Pinto, A. C.
Rafael, Míriam Silva
Keywords: Insecticide
Unclassified Drug
1,3 Dioxolane Derivative
Cytochrome P450
Dill Apiole
Glutathione Transferase
Insect Proteins
Aedes Aegypti
Animals Experiment
Arthropod Larva
Pest Control, Biological
Concentration (parameters)
Controlled Study
Cyp6n12 Gene
Down Regulation
Gene Expression Regulation
Gste7 Gene
Insect Resistance
Insecticide Resistance
Metabolic Stress
Reverse Transcription Polymerase Chain Reaction
Toxicity Testing
Drug Effects
Cytochrome P-450 Enzyme System
Glutathione Transferase
Insect Proteins
Insecticide Resistance
Issue Date: 2015
metadata.dc.publisher.journal: Genetics and Molecular Research
metadata.dc.relation.ispartof: Volume 14, Número 4, Pags. 16728-16735
Abstract: The yellow fever mosquito Aedes (Stegomyia) aegypti is the main vector of dengue arbovirus and other arboviruses. Dengue prevention measures for the control of A. aegypti involve mainly the use of synthetic insecticides. The constant use of insecticides has caused resistance in this mosquito. Alternative studies on plant extracts and their products have been conducted with the aim of controlling the spread of the mosquito. Dillapiole is a compound found in essential oils of the plant Piper aduncum (Piperaceae) which has been effective as a biopesticide against A. aegypti. Isodillapiole is a semisynthetic substance obtained by the isomerization of dillapiole. In the present study, isodillapiole was evaluated for its potential to induce differential expression of insecticide resistance genes (GSTE7 and CYP6N12) in 3rd instar larvae of A. aegypti. These larvae were exposed to this compound at two concentrations (20 and 40 μg/mL) for 4 h during four generations (G1, G2, G3, and G4). Quantitative RT-PCR was used to assess the expression of GSTE7 and CYP6N12 genes. GSTE7 and CYP6N12 relative expression levels were higher at 20 than at 40 μg/mL and varied among generations. The decrease in GSTE7 and CYP6N12 expression levels at the highest isodillapiole concentration suggests that larvae may have suffered from metabolic stress, revealing a potential alternative product in the control of A. aegypti. © FUNPEC-RP.
metadata.dc.identifier.doi: 10.4238/2015.December.11.20
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