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Title: Molecular identification of Amazonian stingless bees using polymerase chain reaction single-strand conformation polymorphism
Authors: Souza, M T
Carvalho-zilse, Gislene Almeida
Keywords: Dna, Mitochondrial
Molecular Marker
Cytochrome C Oxidase
Dna, Mitochondrial
Rna 16s
Coi Gene
Cost Effectiveness Analysis
Dna Polymorphism
Genetic Distance
Genetic Identification
Melipona Dubia
Melipona Eburnea
Melipona Interrupta
Melipona Nebulosa
Melipona Rufiventris
Melipona Seminigra
Polymerase Chain Reaction
Rna 16s Gene
Scaptotrigona Polysticta
Single Strand Conformation Polymorphism
Species Differentiation
Species Identification
Stingless Bee
Taxonomic Identification
Trigona Williana
Polymorphism, Genetic
Evolution, Molecular
Polymerase Chain Reaction
Genetics, Population
Single Strand Conformation Polymorphism
Dna, Mitochondrial
Electron Transport Complex Iv
Evolution, Molecular
Genetics, Population
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Single-stranded Conformational
Rna, Ribosomal, 16s
Issue Date: 2014
metadata.dc.publisher.journal: Genetics and Molecular Research
metadata.dc.relation.ispartof: Volume 13, Número 3, Pags. 5507-5513
Abstract: In countries containing a mega diversity of wildlife, such as Brazil, identifying and characterizing biological diversity is a continuous process for the scientific community, even in face of technological and scientific advances. This activity demands initiatives for the taxonomic identification of highly diverse groups, such as stingless bees, including molecular analysis strategies. This type of bee is distributed in all of the Brazilian states, with the highest species diversity being found in the State of Amazônia. However, the estimated number of species diverges among taxonomists. These bees are considered the main pollinators in the Amazon rainforest, in which they obtain food and shelter; however, their persistence is constantly threatened by deforestation pressure. Hence, it is important to classify the number and abundance of bee specie, to measure their decline and implement meaningful, priority conservation strategies. This study aims to maximize the implementation of more direct, economic and successful techniques for the taxonomic identification of stingless bees. Specifically, the genes 16S rRNA and COI from mitochondrial DNA were used as molecular markers to differentiate 9 species of Amazonian stingless bees based on DNA polymorphism, using the polymerase chain reaction-single-strand conformation polymorphism technique. We registered different, exclusive SSCP haplotypes for both genes in all species analyzed. These results demonstrate that SSCP is a simple and cost-effective technique that is applicable to the molecular identification of stingless bee species. © FUNPEC-RP.
metadata.dc.identifier.doi: 10.4238/2014.July.25.4
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