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Title: Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum
Authors: Cordeiro, Isabelle Bezerra
Castro, Diogo Pereira de
Nogueira, P. P O
Angelo, P.
Nogueira, Paulo Afonso
Gonçalves, José Francisco de Carvalho
Pereira, A. M.R.F.
Garcia, Jerusa Simone
Souza, Gustavo H.M.F.
Arruda, Marco Aurélio Zezzi
Eberlin, M. N.
Astolfi-Filho, Spártaco A.T.
Andrade, E. V. de
López-Lozano, Jorge Luis
Keywords: Bacterial Protein
Carrier Protein
Elongation Factor
Elongation Factor P
Membrane Protein
Ribosome Protein
Sigma Factor
Sigma Factor 32
Small Heat Shock Protein
Superoxide Dismutase
Unclassified Drug
Bacterial Protein
Bacterial Growth
Chromobacterium Violaceum
Heat Acclimatization
Heat-shock Response
Heat Stress
Inverted Repeat
Mass Spectrometry
Promoter Region
Protein Expression
Cell Respiration
Gene Expression Regulation
Growth, Development And Aging
Open Reading Frame
Stress, Physiological
Bacteria (microorganisms)
Chromobacterium Violaceum
Adaptation, Biological
Bacterial Proteins
Cell Respiration
Gene Expression Regulation, Bacterial
Molecular Chaperones
Open Reading Frames
Promoter Regions, Genetic
Stress, Physiological
Issue Date: 2013
metadata.dc.publisher.journal: Genetics and Molecular Research
metadata.dc.relation.ispartof: Volume 12, Número 4, Pags. 5057-5071
Abstract: Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry. The total number of spots detected was 1985; the number ranged from 99 to 380 in each assay. The proteins that were identified spectrometrically were categorized as chaperones, proteins expressed exclusively under heat stress, enzymes involved in the respiratory and fermentation cycles, ribosomal proteins, and proteins related to transport and secretion. Controlling inverted repeat of chaperone expression and inverted repeat DNA binding sequences, as well as regions recognized by sigma factor 32, elements involved in the genetic regulation of the bacterial stress response, were identified in the promoter regions of several of the genes coding proteins, involved in the C. violaceum stress response. We found that 30°C is the optimal growth temperature for C. violaceum, whereas 25, 35, and 40°C are stressful temperatures that trigger the expression of chaperones, superoxide dismutase, a probable small heat shock protein, a probable phasing, ferrichrome-iron receptor protein, elongation factor P, and an ornithine carbamoyltransferase catabolite. This information improves our comprehension of the mechanisms involved in stress adaptation by C. violaceum. © FUNPEC-RP.
metadata.dc.identifier.doi: 10.4238/2013.October.29.1
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