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Título: | Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum |
Autor: | Cordeiro, Isabelle Bezerra Castro, Diogo Pereira de Nogueira, P. P O Angelo, P. Nogueira, Paulo Afonso Gonçalves, José Francisco de Carvalho Pereira, A. M.R.F. Garcia, Jerusa Simone Souza, Gustavo H.M.F. Arruda, Marco Aurélio Zezzi Eberlin, M. N. Astolfi-Filho, Spártaco A.T. Andrade, E. V. de López-Lozano, Jorge Luis |
Palavras-chave: | Bacterial Protein Carrier Protein Chaperone Elongation Factor Elongation Factor P Membrane Protein Ribosome Protein Sigma Factor Sigma Factor 32 Small Heat Shock Protein Superoxide Dismutase Unclassified Drug Bacterial Protein Chaperone Bacterial Growth Chromobacterium Violaceum Electrophoresis Heat Acclimatization Heat-shock Response Heat Stress Inverted Repeat Mass Spectrometry Nonhuman Promoter Region Protein Expression Proteomics Adaptation Cell Respiration Chromobacterium Fermentation Gene Expression Regulation Genetics Growth, Development And Aging Metabolism Open Reading Frame Stress, Physiological Procedures Proteomics Temperature Bacteria (microorganisms) Chromobacterium Violaceum Negibacteria Proteobacteria Adaptation, Biological Bacterial Proteins Cell Respiration Chromobacterium Fermentation Gene Expression Regulation, Bacterial Molecular Chaperones Open Reading Frames Promoter Regions, Genetic Proteomics Stress, Physiological Temperature |
Data do documento: | 2013 |
Revista: | Genetics and Molecular Research |
É parte de: | Volume 12, Número 4, Pags. 5057-5071 |
Abstract: | Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry. The total number of spots detected was 1985; the number ranged from 99 to 380 in each assay. The proteins that were identified spectrometrically were categorized as chaperones, proteins expressed exclusively under heat stress, enzymes involved in the respiratory and fermentation cycles, ribosomal proteins, and proteins related to transport and secretion. Controlling inverted repeat of chaperone expression and inverted repeat DNA binding sequences, as well as regions recognized by sigma factor 32, elements involved in the genetic regulation of the bacterial stress response, were identified in the promoter regions of several of the genes coding proteins, involved in the C. violaceum stress response. We found that 30°C is the optimal growth temperature for C. violaceum, whereas 25, 35, and 40°C are stressful temperatures that trigger the expression of chaperones, superoxide dismutase, a probable small heat shock protein, a probable phasing, ferrichrome-iron receptor protein, elongation factor P, and an ornithine carbamoyltransferase catabolite. This information improves our comprehension of the mechanisms involved in stress adaptation by C. violaceum. © FUNPEC-RP. |
DOI: | 10.4238/2013.October.29.1 |
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