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https://repositorio.inpa.gov.br/handle/1/14993| Title: | Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
| Authors: | Cruz-Hernández, Pedro Batista, Jacqueline da Silva Formiga, Kyara M. Rafael, Míriam Silva Tadei, Wanderli Pedro Santos, Joselita Maria M. |
| Keywords: | Genomic Dna Allele Anopheles Anopheles Triannulatus Dna Hybridization Gene Amplification Gene Locus Genetic Linkage Heterozygosity Microsatellite Marker Nonhuman Nucleotide Sequence Polymorphic Dna Microsatellite Locus Genetics, Population Alleles Animal Anopheles Dna Genetics, Population Humans Linkage Disequilibrium Microsatellite Repeats Polymorphism, Genetic Species Specificity |
| Issue Date: | 2013 |
| metadata.dc.publisher.journal: | Genetics and Molecular Research |
| metadata.dc.relation.ispartof: | Volume 12, Número 3, Pags. 3088-3092 |
| Abstract: | Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed. These loci were used in 4 congeneric species and provide a useful tool for studying population genetics and other aspects of the biology of this and other Anopheles species. ©FUNPEC-RP. |
| metadata.dc.identifier.doi: | 10.4238/2013.March.13.11 |
| Appears in Collections: | Artigos |
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