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Title: Distribution of mating-type alleles and M13 PCR markers in the black leaf spot fungus Mycosphaerella fijiensis of bananas in Brazil
Authors: Queiroz, Casley B.
Miranda, E. C.
Hanada, Rogério Eiji
Sousa, Nelcimar Reis
Gasparotto, Luadir
Soares, Marcos A.
Silva, G. F.
Keywords: Bacteriophage Dna
Fungal Protein
Microsatellite Dna
Controlled Study
Dna Fingerprinting
Fungal Gene
Fungal Plant Disease
Fungus Isolation
Gene Sequence
Genetic Distance
Genetic Marker
Polymorphism, Genetic
Genetic Similarity
Genetic Variability
Geographic Origin
Inter Simple Sequence Repeat
Mat1 1 Gene
Mat1 2 Gene
Mating Type
Mycosphaerella Fijiensis
Plant Leaf
Polymerase Chain Reaction
Species Distribution
Bacteriophage M13
Fungal Gene
Plant Disease
Variable Number Of Tandem Repeat
Bacteriophage M13
Fungal Proteins
Genes, Mating Type, Fungal
Genetic Markers
Microsatellite Repeats
Minisatellite Repeats
Plant Diseases
Plant Leaves
Polymerase Chain Reaction
Polymorphism, Genetic
Issue Date: 2013
metadata.dc.publisher.journal: Genetics and Molecular Research
metadata.dc.relation.ispartof: Volume 12, Número 1, Pags. 443-452
Abstract: The fungus Mycosphaerella fijiensis is the causative agent of black sigatoka, which is one of the most destructive diseases of banana plants. Infection with this pathogen results in underdeveloped fruit, with no commercial value. We analyzed the distribution of the M. fijiensis mating-type system and its genetic variability using M13 phage DNA markers. We found a 1:1 distribution of mating-type alleles, indicating MAT1-1 and MAT1-2 idiomorphs. A polymorphism analysis using three different primers for M13 markers showed that only the M13 minisatellite primers generated polymorphic products. We then utilized this polymorphism to characterize 40 isolates from various Brazilian states. The largest genetic distances were found between isolates from the same location and between isolates from different parts of the country. Therefore, there was no correlation between the genetic similarity and the geographic origin of the isolates. The M13 marker was used to generate genetic fingerprints for five isolates; these fingerprints were compared with the band profiles obtained from inter-simple sequence repeat (UBC861) and inter-retrotransposon amplified polymorphism analyses. We found that the M13 marker was more effective than the other two markers for differentiating these isolates. © FUNPEC-RP.
metadata.dc.identifier.doi: 10.4238/2013.February.8.9
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