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https://repositorio.inpa.gov.br/handle/1/14997| Título: | Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species. |
| Autor: | Guimarães-Marques, Giselle Moura Batista, Jacqueline da Silva Guimarães, H. M. Naice-Daou, M. V. Lima, M. P. Formiga, Kyara M. Santos, Joselita Maria M. Lima, A. C. Rafael, Míriam Silva |
| Palavras-chave: | Microsatellite Dna Animals Anopheles Disease Carrier Dna Sequence Gene Gene Locus Genetics Genotype Polymerase Chain Reaction Species Difference Animal Anopheles Genes, Insect Genetic Loci Genotype Insect Vectors Microsatellite Repeats Polymerase Chain Reaction Sequence Analysis, Dna Species Specificity |
| Data do documento: | 2012 |
| Revista: | Genetics and Molecular Research |
| É parte de: | Volume 11, Número 4, Pags. 4198-4203 |
| Abstract: | The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species. |
| DOI: | 10.4238/2012.December.6.3 |
| Aparece nas coleções: | Artigos |
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