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Title: Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
Authors: Guimarães-Marques, Giselle Moura
Batista, Jacqueline da Silva
Guimarães, H. M.
Naice-Daou, M. V.
Lima, M. P.
Formiga, Kyara M.
Santos, Joselita Maria M.
Lima, A. C.
Rafael, Míriam Silva
Keywords: Microsatellite Dna
Disease Carrier
Dna Sequence
Gene Locus
Polymerase Chain Reaction
Species Difference
Genes, Insect
Genetic Loci
Insect Vectors
Microsatellite Repeats
Polymerase Chain Reaction
Sequence Analysis, Dna
Species Specificity
Issue Date: 2012
metadata.dc.publisher.journal: Genetics and Molecular Research
metadata.dc.relation.ispartof: Volume 11, Número 4, Pags. 4198-4203
Abstract: The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species.
metadata.dc.identifier.doi: 10.4238/2012.December.6.3
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