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Title: | Post-transcriptional silencing of the SGE1 gene induced by a dsRNA hairpin in Fusarium oxysporum f. Sp cubense, the causal agent of Panama disease |
Authors: | Fernandes, J. S. Angelo, C. S. Cruz, J. C. Santos, Jousielle Márcia dos Sousa, Nelcimar Reis Silva, G. F. |
Keywords: | Banana Controlled Study Double Stranded Rna Hairpin Down Regulation Fungal Gene Fungal Strain Fungal Virulence Fungus Culture Fusarium Wilt Gene Expression Regulation Gene Function Gene Identification Gene Induction Genetic Transformation Nonhuman Plant Structures Posttranscriptional Gene Silencing Pseudostem Rhizome Rna Interference Rna Structure Sge1 Gene Sporogenesis Wild Type Fusarium Genetics Metabolism Pathogenicity Virulence Fungal Protein Rna, Small Interfering Fungal Proteins Fusarium Rna Interference Rna, Small Interfering Virulence |
Issue Date: | 2016 |
metadata.dc.publisher.journal: | Genetics and Molecular Research |
metadata.dc.relation.ispartof: | Volume 15, Número 2 |
Abstract: | Fusarium oxysporum f. sp cubense (Foc), the causal agent of Panama disease, is responsible for economic losses in banana crops worldwide. The identification of genes that effectively act on pathogenicity and/or virulence may contribute to the development of different strategies for disease control and the production of resistant plants. The objective of the current study was to analyze the importance of SGE1 gene expression in Foc virulence through post-transcriptional silencing using a double-stranded RNA hairpin. Thirteen transformants were selected based on different morphological characteristics, and sporulation in these transformants was significantly reduced by approximately 95% (P < 0.05) compared to that of the wild-type strain. The relative SGE1 expression levels in the transformant strains were reduced by 27 to 47% compared to those in the wild-type strain. A pathogenicity analysis revealed that the transformants were able to reach the rhizomes and pseudostems of the inoculated banana plants. However, the transformants induced initial disease symptoms in the banana plants approximately 10 days later than that by the wild-type Foc, and initial disease symptoms persisted even at 45 days after inoculation. These results indicate that the SGE1 gene is directly involved in the virulence of Foc. Therefore, SGE1 may be a potential candidate for host-induced gene silencing in banana plants. © FUNPEC-RP. |
metadata.dc.identifier.doi: | 10.4238/gmr.15027941 |
Appears in Collections: | Artigos |
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