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Title: Responses to hypoxia and recovery: Repayment of oxygen debt is not associated with compensatory protein synthesis in the Amazonian cichlid, Astronotus ocellatus
Authors: Lewis, Johanne Mari
Costa, Isabel A.S.F.
Val, Adalberto Luis
Almeida-Val, Vera Maria Fonseca
Gamperl, A. Kurt
Driedzic, William Robert
Keywords: Lactic Acid
Antibody Specificity
Oxygen Consumption
Protein Synthesis
Lactic Acid
Organ Specificity
Oxygen Consumption
Protein Biosynthesis
Astronotus Ocellatus
Issue Date: 2007
metadata.dc.publisher.journal: Journal of Experimental Biology
metadata.dc.relation.ispartof: Volume 210, Número 11, Pags. 1935-1943
Abstract: Oxygen consumption, as an indicator of routine metabolic rate (RoMR), and tissue-specific changes in protein synthesis, as measured by 3H-labelled phenylalanine incorporation rates, were determined in Astronotus ocellatus to investigate the cellular mechanisms behind hypoxia-induced metabolic depression and recovery. RoMR was significantly depressed, by approximately 50%, when dissolved oxygen levels reached 10% saturation (0.67±0.01 mg l-1 at 28±1°C). This depression in RoMR was accompanied by a 50-60% decrease in liver, heart and gill protein synthesis, but only a 30% decrease in brain protein synthesis. During recovery from hypoxia, an overshoot in RoMR to 270% of the normoxic rate was observed, indicating the accumulation of an oxygen debt during hypoxia. This conclusion was consistent with significant increase in plasma lactate levels during the hypoxic exposure, and the fact that lactate levels rapidly returned to pre-hypoxic levels. In contrast, a hyperactivation of protein synthesis did not occur, suggesting the overshoot in oxygen consumption during recovery is attributed to an increase in cellular processes other than protein synthesis.
metadata.dc.identifier.doi: 10.1242/jeb.005371
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