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https://repositorio.inpa.gov.br/handle/1/15158| Título: | Improvement of a PCR test to diagnose infection by Mansonella ozzardi |
| Título(s) alternativo(s): | Adequação da técnica da PCR para diagnóstico de infecção de Mansonella ozzardi |
| Autor: | Vera, Luana Janaína Souza Basano, Sérgio de Almeida Camargo, Juliana de Souza Almeida Aranha França, Andonai Krauze de Godoi Matos Ferreira, Ricardo de Casseb, Almeida Andrade Medeiros, Jansen Fernandes de Fontes, Gilberto Camargo, Luis Marcelo Aranha |
| Palavras-chave: | Blood Smear Clinical Clinical Effectiveness Controlled Study Cost Effectiveness Analysis Diagnostic Test Diagnostic Test Accuracy Study Giemsa Stain Gold Standard Human Intermethod Comparison Mansonella Ozzardi Mansonelliasis Membrane Filter Nonhuman Nucleotide Sequence Performance Measurement System Polycarbonate Membrane Filter Polymerase Chain Reaction Predictive Value Sensitivity And Specificity Mansonella Ozzardi Nucleopolyhedrovirus |
| Data do documento: | 2011 |
| Revista: | Revista da Sociedade Brasileira de Medicina Tropical |
| É parte de: | Volume 44, Número 3, Pags. 380-382 |
| Abstract: | Introduction: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. Te common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. Methods: In order to increase diagnosis efcacy, the PCR technique was improved. Results and Conclusions: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood fltration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears. |
| DOI: | 10.1590/S0037-86822011000300023 |
| Aparece nas coleções: | Artigos |
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