Use este identificador para citar ou linkar para este item: https://repositorio.inpa.gov.br/handle/1/15224
Título: PH drop impacts differentially skin and gut microbiota of the Amazonian fish tambaqui (Colossoma macropomum)
Autor: Sylvain, François Étienne
Cheaib, Bachar
Llewellyn, Martin Stephen
Correia, Tiago Gabriel
Fagundes, Daniel Barros
Val, Adalberto Luis
Derôme, Nicolas
Palavras-chave: Rna 16s
Animals
Bacterium
Betaproteobacteria
Characiformes
Chemistry
Classification
Genetics
Gill
Intestine Flora
Isolation And Purification
Metabolism
Microbiology
Ph
Sequence Analysis
Skin
Animal
Bacteria
Betaproteobacteria
Characiformes
Gastrointestinal Microbiome
Gills
Hydrogen-ion Concentration
Rna, Ribosomal, 16s
Sequence Analysis, Rna
Skin
Data do documento: 2016
Revista: Scientific Reports
É parte de: Volume 6
Abstract: Aquatic organisms are increasingly exposed to lowering of environmental pH due to anthropogenic pressure (e.g. acid rain, acid mine drainages). Such acute variations trigger imbalance of fish-Associated microbiota, which in turn favour opportunistic diseases. We used the tambaqui (Colossoma macropomum), an Amazonian fish tolerant to significant pH variation in its natural environment, to assess the response of fish endogenous microbiota to acute short-Term acid stress. We exposed 36 specimens of tambaquis to acidic water (pH 4.0) over 2 consecutive weeks and sampled cutaneous mucus, feces and water at 0, 7 &14 days. The 16S RNA hypervariable region V4 was sequenced on Illumina MiSeq. After two weeks of acidic exposure, fecal and skin microbiota taxonomic structures exhibited different patterns: skin microbiota was still exhibiting a significantly disturbed composition whereas fecal microbiota recovered a similar composition to control group, thus suggesting a stronger resilience capacity of the intestinal microbiota than cutaneous microbiota.
DOI: 10.1038/srep32032
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