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Title: In vitro antibiotic and modulatory activity of Mesosphaerum suaveolens (L.) kuntze against candida strains
Authors: Costa, Adrielle Rodrigues
Bezerra, José Weverton Almeida
Cruz, Rafael Pereira da
Freitas, Maria Audilene de
Silva, Viviane Bezerra da
Neto, João Cruz
dos Santos, Antônia Thassya Lucas
Braga, Maria Flaviana Bezerra Morais
Silva, Leomara Andrade da
Rocha, Maria Ivaneide
Kamdem, J. P.
Iriti, Marcello
Vitalini, Sara
Duarte, Antônia Eliene
Barros, Luiz Marivando
Keywords: Antibiotic Agent
Caffeic Acid
Chlorogenic Acid
Ellagic Acid
Gallic Acid
Mesosphaerum Suaveolens Extract
Phenol Derivative
Plant Extract
Unclassified Drug
Anti-fungal Activity
Antifungal Resistance
Anti-microbial Activity
Broth Dilution
Candida Tropicalis
Cell Viability
Cell Viability Assay
Controlled Study
Disk Diffusion
Drug Activity
Enzyme-linked Immunosorbent Assay
Fungal Strain
Fungus Growth
High Performance Liquid Chromatography
Ic 50
Medicinal Plant
Mesosphaerum Suaveolens
Minimum Fungicidal Concentration
Minimum Inhibitory Concentration
Priority Journal
Retention Time (chromatography)
Ultraviolet Spectroscopy
Issue Date: 2020
metadata.dc.publisher.journal: Antibiotics
metadata.dc.relation.ispartof: Volume 9, Número 2
Abstract: The emergence of fungal resistance to commercial drugs has been a major problem for the WHO. In this context, research with natural products is promising in the discovery of new active substances. Thus, this work evaluated the antifungal effect of a medicinal plant (i.e., Mesosphaerum suaveolens) against strains of the genus Candida, tested the combined effect with the drug fluconazole, and, finally, determined the phenolic constituents present in the species. Initially, aqueous extracts of leaves (AELMs) and aerial parts (AEAPMs) of the species were prepared. For microbiological assays, the minimum fungicidal concentration was determined by broth microdilution, and the combined effect of fluconazole extracts were verified by sub‐inhibitory microdilution concentrations (CFM/8) followed by spectrophotometric readings which were used to determine the IC50. HPLC detected the presence of flavonoids and phenolic acids, detecting eight compounds present in the samples of which caffeic acid and quercetin were major components. The AELMs modulated fluconazole activity since it decreased fluconazole’s IC50 from 7.8 μg/mL to an IC50 of 4.7 μg/mL (CA LM 77) and from 28.8 μg/mL to 18.26 μg/mL (CA INCQS 40006) for the C. albicans strains. The AEAPMs were able to potentiate the effect of fluconazole more effectively than the AELMs. Such an effect was significant for the 16 μg/mL concentration for CA LM 77 and 32 μg/mL for CA INCQS 40006. The AEAPMs as well as the AELMs presented clinically relevant activities for C. tropicalis strains. For the C. tropicalis LM 23 strain, the AEPMs obtained an IC50 of 25 μg/mL and the AELMs an IC50 of 359.9 μg/mL. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
metadata.dc.identifier.doi: 10.3390/antibiotics9020046
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