Use este identificador para citar ou linkar para este item: https://repositorio.inpa.gov.br/handle/1/15482
Título: Natural infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a transition area of north-northeast Brazil
Autor: Aragão, Carine Fortes
Pinheiro, Valéria Cristina Soares
Neto, Joaquim Pinto Nunes
Silva, Eliana Vieira Pinto da
Pereira, Glennda Juscely Galvão
Nascimento, Bruna Laís Sena do
Castro, Karoline da Silva
Maia, Ariadne Mendonça
Catete, Clístenes Pamplona
Martins, Lívia Carício S.
Tadei, Wanderli Pedro
Silva, Sandro Patroca da
Cruz, Ana Cecília Ribeiro
Palavras-chave: Virus Rna
Aedes Aegypti
Aedes Albopictus
Chikungunya
Chikungunya Virus
Controlled Study
Dengue
Dengue Virus 2
Female
Microscopy, Fluorescence
Gene Sequence
Genotype
Immunofluorescence Test
Male
Nonhuman
Nucleotide Sequence
Phylogenetic Tree
Phylogeny
Real-time Polymerase Chain Reaction
Reverse Transcription Polymerase Chain Reaction
Sequence Alignment
Virus Isolation
Virus Load
Virus Transmission
Zika Fever
Zika Virus
Data do documento: 2019
Revista: Viruses
É parte de: Volume 11, Número 12
Abstract: Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya viruses in Ae. aegypti and Ae. albopictus from the municipalities of Alto Alegre, Caxias, Codó, and São Mateus do Maranhão, located in the state of Maranhão, Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, and then submitted to RNA extraction and RT-qPCR. The positive samples were confirmed by virus isolation and genome sequencing. Three hundred and forty-eight Ae. aegypti (176 males and 172 females) and 12 Ae. albopictus (eight males and four females) were collected and tested. Ae. aegypti was the only vector positive in two municipalities-Codó, with detection of Chikungunya virus (CHIKV) belonging to the East-Central-South African genotype, and in Caxias, with detection of Dengue virus (DENV)-2 belonging to the Asian/American genotype. The detection of CHIKV and DENV-2 is evidence that those viruses are maintained in arthropod vectors, and shows the epidemiological risk in the area for chikungunya cases and a possible increase of severe dengue cases, associated with the occurrence of dengue hemorrhagic fever. © 2019 by the authors.
DOI: 10.3390/v11121126
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