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Title: Lipolytic Enzymes with Hydrolytic and Esterification Activities Produced by Filamentous Fungi Isolated from Decomposition Leaves in an Aquatic Environment
Authors: Mendes, Danylo Bezerra
Silva, F. F.da
Guarda, Patrícia Martins
Almeida, Alex Fernando de
Oliveira, Deyla Paula de
Morais, Paula Benevides de
Guarda, Emerson A.
Keywords: Agar
Fungal Enzyme
Olive Oil
Organic Nitrogen
Polysorbate 20
Rhodamine B
Triacylglycerol Lipase
Vegetable Oil
Aquatic Environment
Controlled Study
Enzyme Synthesis
Filamentous Fungus
Fungus Culture
Fungus Growth
Fungus Isolation
Fusarium Solani
Hypocrea Lixii
Plant Leaf
Issue Date: 2019
metadata.dc.publisher.journal: Enzyme Research
metadata.dc.relation.ispartof: Volume 2019
Abstract: Microbial lipases are prominent biocatalysts able to catalyze a wide variety of reactions in aqueous and nonaqueous media. In this work, filamentous fungi isolated from leaves decomposed in an aquatic environment were screened for lipase production with hydrolytic activity and esterification. Agar plates with Tween 20 and Rhodamine B were used for selection, while submerged cultures with olive oil were subsequently used to select 38 filamentous fungi. Trichoderma harzianum, Fusarium solani, Trichoderma harzianum F5, and Penicillium sp. F36 were grown in six different culture media. F. solani presented the highest lipase production (2.37 U/mL) with esterification activity of 0.07 U/mL using medium composed of (g.L-1) KH2PO4 1.00, MgSO4 H2O 1.123, and CuSO4 0.06. Supplementation of this culture medium with organic nitrogen sources increased lipase production by 461.3% using tryptone and by 419.4% using yeast extract. Among the vegetable oils from the Amazon region, degummed cotton oil induced lipase production up to 8.14 U/mL. The lipase produced by F. solani F61 has great potential to application in conventional processes and biodiesel production by transesterification of vegetable oils, as well as food industries in the production of fatty acid esters by hydrolysis and esterification. © 2019 D. B. Mendes et al.
metadata.dc.identifier.doi: 10.1155/2019/8182425
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