Use este identificador para citar ou linkar para este item: https://repositorio.inpa.gov.br/handle/1/15596
Título: Zerumbone from Zingiber zerumbet (L.) smith: A potential prophylactic and therapeutic agent against the cariogenic bacterium Streptococcus mutans
Autor: Moreira da Silva, Thiago
Pinheiro, Carlos Danniel
Orlandi, Patrícia Puccinelli
Pinheiro, Carlos Cleomir S.
Pontes, Gemilson Soares
Palavras-chave: Essential Oil
Plant Extract
Unclassified Drug
Zerumbone
Zingiber Zerumbet Extract
Antiinfective Agent
Essential Oil
Sesquiterpenes
Zerumbone
Animals Cell
Anti-microbial Activity
Bacterial Strain
Biological Activity
Concentration Response
Controlled Study
Crystallization
Cytotoxicity
Dental Caries
Dental Prevention
Dilution
Drug Determination
Drug Isolation
Drug Purification
Drug Purity
High Performance Liquid Chromatography
Hydrodistillation
Minimum Bactericidal Concentration
Minimum Inhibitory Concentration
Mtt Assay
Nonhuman
Rhizome
Streptococcus Mutans
Zingiber Zerumbet
Chemistry
Drug Effect
Isolation And Purification
Microbial Sensitivity Test
Microbial Viability
Streptococcus Mutans
Zingiberaceae
Anti-bacterial Agents
Microbial Sensitivity Tests
Microbial Viability
Oils, Volatile
Sesquiterpenes
Streptococcus Mutans
Zingiberaceae
Data do documento: 2018
Revista: BMC Complementary and Alternative Medicine
É parte de: Volume 18, Número 1
Abstract: Background: Essential oil obtained from rhizomes of the Zingiber zerumbet (L.) Smith (popularly known in Brazil as bitter ginger) is mainly constituted by the biomolecule zerumbone, which exhibit untapped antimicrobial potential. The aim of this study was to investigate the antimicrobial activity of the zerumbone from bitter ginger rhizomes against the cariogenic agent Streptococcus mutans. Methods: Firstly, the essential oil from rhizomes of Zingiber zerumbet (L.) Smith extracted by hydrodistillation was submitted to purification and recrystallization process to obtain the zerumbone compound. The purity of zerumbone was determined through high-performance liquid chromatography analysis. Different concentrations of zerumbone were tested against the standard strain S. mutans (ATCC 35668) by using microdilution method. The speed of cidal activity was determined through a time kill-curve assay. The biological cytotoxicity activity of zerumbone was assessed using Vero cell line through MTT assay. Results: The zerumbone showed a minimum inhibitory concentration (MIC) of 250 μg/mL and a minimum bactericidal concentration (MBC) of 500 μg/mL against S. mutans. After six hours of bacteria-zerumbone interaction, all concentrations tested starts to kill the bacteria and all bacteria were killed between 48 and 72 h period at the concentration of 500 μg/mL (99,99% of bacteria were killed in comparison with original inoculum). In addition, zerumbone showed no cytotoxicity activity on mammalian continuous cells line. Conclusions: These results draw attention to the potential of zerumbone as antimicrobial agent against S. mutans infection, indicating its possible use in the phyto-pharmaceutical formulations as new approach to prevent and treat tooth decay disease. © 2018 The Author(s).
DOI: 10.1186/s12906-018-2360-0
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