Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/15816
Title: Successful xenogeneic germ cell transplantation from Jundia catfish (Rhamdia quelen) into adult Nile tilapia (Oreochromis niloticus) testes
Authors: Silva, Martinho de Almeida E.
Costa, Guilherme M.J.
Lacerda, Samyra Maria Santos N.Nassif
Brandão-Dias, Pedro Ferreira Pinto
Kalapothakis, Evanguedes
Silva Júnior, Alaion Fonseca da
Alvarenga, Érika Ramos de
França, Luiz Renato de
Keywords: Allotransplantation
Aquaculture
Blastula
Catfish
Cell Viability
Controlled Study
Developmental Stage
Embryo
Endangered Species
Fertilization In Vitro
Gametogenesis
Genetic Analysis
Genetic Marker
Genetic Trait
Germ Cell
Germ Cell Transplantation
Germplasm
Heredity
Laboratory Test
Larva
Male
Nonhuman
Oreochromis Niloticus
Phylogeny
Priority Journal
Progeny
Recipient
Rhamdia Quelen
Seminiferous Tubule
Somatic Cell
Spermatid
Spermatocyte
Spermatogenesis
Spermatogonium
Testis Development
Tilapia
Animals
Cell Transplantation
Cytology
Environmental Protection
Genetics
Growth, Development And Aging
Metabolism
Physiology
Procedures
Spermatozoon
Testis
Xenograft
Animal
Aquaculture
Catfishes
Cell Transplantation
Conservation Of Natural Resources
Endangered Species
Heterografts
Male
Seminiferous Tubules
Spermatids
Spermatocytes
Spermatogenesis
Spermatogonia
Spermatozoa
Testis
Tilapia
Transplantation, Heterologous
Issue Date: 2016
metadata.dc.publisher.journal: General and Comparative Endocrinology
metadata.dc.relation.ispartof: Volume 230-231, Pags. 48-56
Abstract: Fish germ cell transplantation presents several important potential applications for aquaculture, including the preservation of germplasm from endangered fish species with high genetic and commercial values. Using this technique in studies developed in our laboratory with adult male Nile tilapias (Oreochromis niloticus), all the necessary procedures were successfully established, allowing the production of functional sperm and healthy progeny approximately 2 months after allogeneic transplantation. In the present study, we evaluated the viability of the adult Nile tilapia testis to generate sperm after xenogeneic transplant of germ cells from sexually mature Jundia catfish (Rhamdia quelen) that belong to a different taxonomic order. Therefore, in order to investigate at different time-periods post-transplantation, the presence and development of donor PKH26 labeled catfish germ cells were followed in the tilapia seminiferous tubules. From 7 to 20 days post-transplantation, only PKH26 labeled spermatogonia were observed, whereas spermatocytes at different stages of development were found at 70 days. Germ cell transplantation success and progression of spermatogenesis were indicated by the presence of labeled PKH26 spermatids and sperm on days 90 and 120 post-transplantation, respectively. Confirming the presence of the catfish genetic material in the tilapia testis, all recipient tilapias evaluated (n = 8) showed the genetic markers evaluated. Therefore, we demonstrated for the first time that the adult Nile tilapia testis offers the functional conditions for development of spermatogenesis with sperm production from a fish species belonging to a different order, which provides an important new venue for aquaculture advancement. © 2016 Elsevier Inc.
metadata.dc.identifier.doi: 10.1016/j.ygcen.2016.03.012
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