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https://repositorio.inpa.gov.br/handle/1/15873
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Campo DC | Valor | Idioma |
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dc.contributor.author | Alves, Ana Cecília | - |
dc.contributor.author | Vasconcelos, Mayron Alves de | - |
dc.contributor.author | Santiago, Mayara Quiroz | - |
dc.contributor.author | Pinto-Junior, Vanir Reis | - |
dc.contributor.author | Osterne, Vinicius José Silva | - |
dc.contributor.author | Lóssio, Cláudia Figueiredo | - |
dc.contributor.author | Souza Ferreira Bringel, Pedro Henrique | - |
dc.contributor.author | Castro, Rondinelle Ribeiro | - |
dc.contributor.author | Nagano, C. S. | - |
dc.contributor.author | Delatorre, Plínio | - |
dc.contributor.author | Souza, Luis Augusto Gomes | - |
dc.contributor.author | Nascimento, K. S. | - |
dc.contributor.author | Assreuy, Ana Maria Sampaio | - |
dc.contributor.author | Cavada, B. S. | - |
dc.date.accessioned | 2020-05-19T20:34:03Z | - |
dc.date.available | 2020-05-19T20:34:03Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | https://repositorio.inpa.gov.br/handle/1/15873 | - |
dc.description.abstract | A novel lectin from seeds of Clathrotropis nitida (CNA) was purified and characterized. CNA is a glycoprotein containing approximately 3.3% carbohydrates in its structure. CNA promoted intense agglutination of rabbit erythrocytes, which was inhibited by galactosides and porcine stomach mucin (PSM). The lectin maintained its hemagglutinating activity after incubation in a wide range of temperatures (30-60 °C) and pH (6.0-7.0), and its binding activity was dependent on divalent cations (Ca+2 and Mg+2). SDS-PAGE showed an electrophoretic profile consisting of a single band of 28 kDa, as confirmed by electrospray ionization mass spectrometry, which indicated an average molecular mass of 27,406 ± 2 Da and the possible presence of isoforms and glycoforms. In addition, CNA exhibited no toxicity to Artemia sp. nauplii and elicited reversible and dose-dependent vasorelaxation in precontracted aortic rings. CNA was successfully immobilized on chitosan beads and was able to capture PSM in solution. This study demonstrated that CNA is a lectin that has potential as a biotechnological tool in glycomics and glycoproteomics applications. © 2015 Elsevier Inc. | en |
dc.language.iso | en | pt_BR |
dc.relation.ispartof | Volume 588, Pags. 33-40 | pt_BR |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Brazil | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/br/ | * |
dc.subject | Calcium | en |
dc.subject | Carbohydrate | en |
dc.subject | Chitosan | en |
dc.subject | Galactoside | en |
dc.subject | Lectin | en |
dc.subject | Magnesium | en |
dc.subject | Stomach Mucin | en |
dc.subject | Immobilized Protein | en |
dc.subject | Plant Lectin | en |
dc.subject | Vasodilator Agent | en |
dc.subject | Animals Cell | en |
dc.subject | Animals Experiment | en |
dc.subject | Animals Model | en |
dc.subject | Binding Affinity | en |
dc.subject | Carbohydrate Analysis | en |
dc.subject | Clathrotropis Nitida | en |
dc.subject | Controlled Study | en |
dc.subject | Dose Response | en |
dc.subject | Electrospray Mass Spectrometry | en |
dc.subject | Glycobiology | en |
dc.subject | Male | en |
dc.subject | Medicinal Plant | en |
dc.subject | Molecular Weight | en |
dc.subject | Nonhuman | en |
dc.subject | Physical Chemistry | en |
dc.subject | Seed Plant | en |
dc.subject | Electrophoresis, Polyacrylamide Gel | en |
dc.subject | Priority Journal | en |
dc.subject | Protein Analysis | en |
dc.subject | Protein Immobilization | en |
dc.subject | Rat | en |
dc.subject | Temperature | en |
dc.subject | Vascular Ring | en |
dc.subject | Vasodilatation | en |
dc.subject | Amino Acid Sequence | en |
dc.subject | Animals | en |
dc.subject | Artemia | en |
dc.subject | Chemistry | en |
dc.subject | Drug Effects | en |
dc.subject | Fabaceae | en |
dc.subject | Genetics | en |
dc.subject | Hemagglutination | en |
dc.subject | Human | en |
dc.subject | In Vitro Study | en |
dc.subject | Isolation And Purification | en |
dc.subject | Molecular Genetics | en |
dc.subject | Physiology | en |
dc.subject | Rabbit | en |
dc.subject | Sequence Homology | en |
dc.subject | Aorta, Thoracic | en |
dc.subject | Wistar Rat | en |
dc.subject | Amino Acid Sequence | en |
dc.subject | Animal | en |
dc.subject | Aorta, Thoracic | en |
dc.subject | Artemia | en |
dc.subject | Chitosan | en |
dc.subject | Fabaceae | en |
dc.subject | Hemagglutination | en |
dc.subject | Humans | en |
dc.subject | Immobilized Proteins | en |
dc.subject | In Vitro Techniques | en |
dc.subject | Male | en |
dc.subject | Molecular Sequence Data | en |
dc.subject | Molecular Weight | en |
dc.subject | Plant Lectins | en |
dc.subject | Plants, Medicinal | en |
dc.subject | Rabbits | en |
dc.subject | Rats | en |
dc.subject | Rats, Wistar | en |
dc.subject | Seeds | en |
dc.subject | Sequence Homology, Amino Acid | en |
dc.subject | Vasodilator Agents | en |
dc.title | A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partial characterization and immobilization in chitosan beads | en |
dc.type | Artigo | pt_BR |
dc.identifier.doi | 10.1016/j.abb.2015.10.020 | - |
dc.publisher.journal | Archives of Biochemistry and Biophysics | pt_BR |
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