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Title: Antibacterial activity and chemical compounds of leaves and branches of Protium hebetatum
Other Titles: Atividade antibacteriana e compostos químicos de folhas e galhos de Protium hebetatum
Authors: Conrado, G. G.
Simplicio, Fernanda Guilhom
Costa, K. R C
Rehder, Vera Lúcia Garcia
Espinar, M. F.
Souza, G. O.
Sampaio, Paulo de Tarso Barbosa
Keywords: Alpha Amyrin
Beta Amyrin
Organic Compound
Plant Extract
Protium Hebetatum Extract
Unclassified Drug
Antibacterial Activity
Broth Dilution
Enterococcus Faecalis
Escherichia Coli
Inhibition Zone
Mass Fragmentography
Minimum Inhibitory Concentration
Plant Leaf
Proteus Vulgaris
Protium Hebetatum
Pseudomonas Aeruginosa
Skin Infection
Staphylococcus Aureus
Chromatography, Thin Layer
Urinary Tract Infection
Issue Date: 2015
metadata.dc.publisher.journal: Revista Brasileira de Plantas Medicinais
metadata.dc.relation.ispartof: Volume 17, Número 4, Pags. 865-874
Abstract: The extracts and fractions of leaves and branches of Protium hebetatum D. C. Daly (Burseraceae) were investigated for their antibacterial activity and chemical composition. The methanol extract of branches (EMG) was considered active against the Escherichia coli and the Proteus vulgaris, showing an inhibition zone of 13 mm, and was selected for bioassayguided phytochemical fractionation. From the technique of broth microdilution, the extract was considered a moderate inhibitor against Staphylococcus aureus, Pseudomonas aeruginosa and Enterococcus faecalis, with a minimum inhibitory concentration (MIC) of 1 mg/mL. The dichloromethane fraction was considered a moderate inhibitor against S. aureus (MIC of 1 mg/mL) and a potent inhibitor against E. faecalis (MIC of 0.5 mg/mL). F1, F2, F5 and F6 from chromatographic column of dichloromethane fraction were considered moderate inhibitors against S. aureus (MIC of 1 mg/mL). Through analysis by a gas chromatography mass spectrometry, eighteen compounds were identified, from which thirteen (isoeugenol, p-vinylguaiacol, metoxyeugenol, coumarin, 5-hydroxy-scopoletin, 4,7-dihydroxy-6-metoxicromam-2-one, 4[(1E]- 3-hydroxy-1-propenyl)-2-methoxyphenol, piperonal, scoparon, o-guaiacol, spathulenol, seringol and antiarol) are unprecedented in these species. We also identified the triterpenes α-amyrin and β-amyrin, the steroids stigmasterol and sitosterol and the coumarin scopoletin, which was closely linked to the antibacterial activity of the samples. © 2015, Instituto de Biociencias. All rights reserved.
metadata.dc.identifier.doi: 10.1590/1983-084X/14_123
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