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dc.contributor.authorScarpassa, Vera Margarete-
dc.contributor.authorAlencar, Ronildo Baiatone-
dc.date.accessioned2020-05-22T18:37:52Z-
dc.date.available2020-05-22T18:37:52Z-
dc.date.issued2013-
dc.identifier.urihttps://repositorio.inpa.gov.br/handle/1/16038-
dc.description.abstractBackground: Lutzomyia umbratilis (a probable species complex) is the main vector of Leishmania guyanensis in the northern region of Brazil. Lutzomyia anduzei has been implicated as a secondary vector of this parasite. These species are closely related and exhibit high morphological similarity in the adult stage; therefore, they have been wrongly identified, both in the past and in the present. This shows the need for employing integrated taxonomy. Methods. With the aim of gathering information on the molecular taxonomy and evolutionary relationships of these two vectors, 118 sequences of 663 base pairs (barcode region of the mitochondrial DNA cytochrome oxidase I - COI) were generated from 72 L. umbratilis and 46 L. anduzei individuals captured, respectively, in six and five localities of the Brazilian Amazon. The efficiency of the barcode region to differentiate the L. umbratilis lineages I and II was also evaluated. The data were analyzed using the pairwise genetic distances matrix and the Neighbor-Joining (NJ) tree, both based on the Kimura Two Parameter (K2P) evolutionary model. Results: The analyses resulted in 67 haplotypes: 32 for L. umbratilis and 35 for L. anduzei. The mean intra-specific genetic distance was 0.008 (0.002 to 0.010 for L. umbratilis; 0.008 to 0.014 for L. anduzei), whereas the mean interspecific genetic distance was 0.044 (0.041 to 0.046), supporting the barcoding gap. Between the L. umbratilis lineages I and II, it was 0.009 to 0.010. The NJ tree analysis strongly supported monophyletic clades for both L. umbratilis and L. anduzei, whereas the L. umbratilis lineages I and II formed two poorly supported monophyletic subclades. Conclusions: The barcode region clearly separated the two species and may therefore constitute a valuable tool in the identification of the sand fly vectors of Leishmania in endemic leishmaniasis areas. However, the barcode region had not enough power to separate the two lineages of L. umbratilis, likely reflecting incipient species that have not yet reached the status of distinct species. © 2013 Scarpassa and Alencar; licensee BioMed Central Ltd.en
dc.language.isoenpt_BR
dc.relation.ispartofVolume 6, Número 1pt_BR
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/br/*
dc.subjectCytochrome C Oxidaseen
dc.subjectDnaen
dc.subjectDna, Mitochondrialen
dc.subjectCladisticsen
dc.subjectDna Barcodingen
dc.subjectDna Extractionen
dc.subjectDna Sequenceen
dc.subjectGenetic Distanceen
dc.subjectHaplotypeen
dc.subjectLeishmaniaen
dc.subjectLeishmaniasisen
dc.subjectLutzomyiaen
dc.subjectLutzomyia Anduzeien
dc.subjectLutzomyia Umbratilisen
dc.subjectNonhumanen
dc.subjectNucleotide Sequenceen
dc.subjectParasite Vectoren
dc.subjectPolymerase Chain Reactionen
dc.subjectTaxonomyen
dc.subjectDipteraen
dc.subjectLeishmania Guyanensisen
dc.subjectLutzomyia Umbratilisen
dc.subjectPhlebotominaeen
dc.subjectPsychodidaeen
dc.subjectAnimalen
dc.subjectDisease Vectorsen
dc.subjectHaplotypesen
dc.subjectLeishmania Guyanensisen
dc.subjectMicroscopyen
dc.subjectMolecular Sequence Dataen
dc.subjectPhylogenyen
dc.subjectPsychodidaeen
dc.subjectSequence Analysis, Dnaen
dc.subjectSequence Homologyen
dc.titleMolecular taxonomy of the two Leishmania vectors Lutzomyia umbratilis and Lutzomyia anduzei (Diptera: Psychodidae) from the Brazilian Amazonen
dc.typeArtigopt_BR
dc.identifier.doi10.1186/1756-3305-6-258-
dc.publisher.journalParasites and Vectorspt_BR
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