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Title: Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey
Authors: Pimentel, Renah Boanerges de Queiroz
Costa, Cristóvão Alves da
Albuquerque, Patrícia Melchionna
Duvoisin Júnior, Sérgio
Keywords: Agar
Phenol Derivative
Agar Diffusion
Antibacterial Activity
Bacterial Growth
Broth Dilution
Controlled Study
Entomopathogenic Bacterium
Escherichia Coli
Gram Negative Bacterium
Gram Positive Bacterium
High Performance Liquid Chromatography
Liquid Liquid Extraction
Proteus Vulgaris
Salmonella Paratyphi
Shigella Sonnei
Staphylococcus Aureus
Stingless Bee
Anti-bacterial Agents
Chromatography, High Pressure Liquid
Gram-negative Bacteria
Gram-positive Bacteria
Issue Date: 2013
metadata.dc.publisher.journal: BMC Complementary and Alternative Medicine
metadata.dc.relation.ispartof: Volume 13
Abstract: Background: Honey has been identified as a potential alternative to the widespread use of antibiotics, which are of significant concern considering the emergence of resistant bacteria. In this context, this study aimed to evaluate the antimicrobial activity of honey samples produced by a stingless bee species and by Apis sp. against pathogenic bacteria, as well as to identify the presence of phenolic compounds.Methods: Honey samples from the stingless bee M. compressipes manaosensis were collected twice, during the dry and rainy seasons. Three commercial honey samples from Apis sp. were also included in this study. Two different assays were performed to evaluate the antibacterial potential of the honey samples: agar-well diffusion and broth macrodilution. Liquid-liquid extraction was used to assess phenolic compounds from honey. HPLC analysis was performed in order to identify rutin and apigenin on honey samples. Chromatograms were recorded at 340 and 290 nm.Results: Two honey samples were identified as having the highest antimicrobial activity using the agar diffusion method. Honey produced by Melipona compressipes manaosensis inhibited the growth of Staphylococcus aureus, Escherichia coli (0157: H7), Proteus vulgaris, Shigella sonnei and Klebsiella sp. A sample of honey produced by Apis sp. also inhibited the growth of Salmonella paratyphi. The macrodilution technique presented greater sensitivity for the antibacterial testing, since all honey samples showed activity. Flavonoid rutin was identified in the honey sample produced by the stingless bee.Conclusions: Honey samples tested in this work showed antibacterial activity against Gram-positive and Gram-negative bacteria. The results reported herein highlight the potential of using honey to control bacterial growth. © 2013 Pimentel et al.; licensee BioMed Central Ltd.
metadata.dc.identifier.doi: 10.1186/1472-6882-13-151
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