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https://repositorio.inpa.gov.br/handle/1/16071
Title: | Triterpenes and the antimycobacterial activity of duroia macrophylla huber (Rubiaceae) |
Authors: | Martins, Daiane Carrion, Lillian Lucas Ramos, Daniela Fernandes Salomé, Kahlil Schwanka Silva, Pedro A. da Barison, Andersson Nunez, C. V. |
Keywords: | Duroia Macrophylla Extract Flavonoid Oleanolic Acid Plant Extract Unclassified Drug Ursolic Acid Antiinfective Agent Phenol Derivative Plant Extract Tannin Derivative Triterpene Antibacterial Activity Carbon Nuclear Magnetic Resonance Drug Isolation Duroia Macrophylla Heteronuclear Multiple Bond Correlation Minimum Inhibitory Concentration Mycobacterium Tuberculosis Nonhuman Phytochemistry Plant Leaf Proton Nuclear Magnetic Resonance Rubiaceae Chemistry Drug Effect Hydrolysis Metabolism Microbial Sensitivity Test Mycobacterium Tuberculosis Nuclear Magnetic Resonance Spectroscopy Reproducibility Anti-bacterial Agents Hydrolysis Magnetic Resonance Spectroscopy Microbial Sensitivity Tests Mycobacterium Tuberculosis Phenols Plant Extracts Plant Leaves Reproducibility Of Results Rubiaceae Tannins Triterpenes |
Issue Date: | 2013 |
metadata.dc.publisher.journal: | BioMed Research International |
metadata.dc.relation.ispartof: | Volume 2013 |
Abstract: | Duroia macrophylla popularly known as "cabeça-de-urubú, " "apuruí," or "puruí-grande-da-mata" occurs in the Amazon Forest. Its leaves and branches were collected twice and extracted with dichloromethane and methanol. All extracts were subjected to phytochemical investigation and terpenes and flavonoids were found in all dichloromethane and methanol extracts, respectively. Methanol extracts from both branches (1st collection) and leaves (2nd collection) presented hydrolyzed tannins, yet alkaloids were only detected in the dichloromethane and methanol extracts from branches at the 2nd collection. Phenol compounds were found in both dichloromethane extracts' collections. The action of every extract was assayed against Mycobacterium tuberculosis (RMPr, H37Rv, and INHr strains), showing that the dichloromethane extract from leaves (1st collection) has the major biological activity, with a MIC of 6.25 μg/mL for the INHr strain, 25.0 μg/mL for the RMPr strain, and ≤6.25 μg/mL for the H37Rv strain. The chromatographic fractioning of the dichloromethane extract from leaves (1st collection) yielded the isolation of two triterpenes: oleanolic and ursolic acids, which were identified by NMR analysis and reported for the first time in the Duroia genus. © 2013 Daiane Martins et al. |
metadata.dc.identifier.doi: | 10.1155/2013/605831 |
Appears in Collections: | Artigos |
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