Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBorges-Moroni, R.-
dc.contributor.authorFraga, Elmary da Costa-
dc.contributor.authorMaia, Juracy de Freitas-
dc.contributor.authorTadei, Wanderli Pedro-
dc.contributor.authorSantos, Joselita Maria M.-
dc.description.abstractOur group analyzed species of Anopheles of the subgenus Nyssorhynchus (An. darlingi, An. marajoara, An. oswaldoi, An. benarrochi, An. triannulatus and An. rangeli) and a single species of the subgenus Anopheles (An. mattogrossensis), originating in the Amazon, based on the mitochondrial DNA control region. The fragment had 381 pb and the nucleotide composition in A+T ranged from 85.8 to 89.5%. DNA polymorphism analysis of the species detected 15 haplotypes, with the presence of 81 polymorphic loci, 95 mutations, haplotypic divergence of 0.879, nucleotide diversity of 0.06507, nucleotide differences pair the pair mean of 23.1749, and the observed and expected variances of 113.840 and 8.33, respectively. The genetic distance among the Anopheles species varied from 0.06 to 1.37%. An. oswaldoi and An. rangeli were the most similar, with nucleotide divergence of 0.17%. The An. benarrochi populations of Ji-Paraná and Bolivia showed nucleotide divergence of 0.06%. An. darlingi, An. marajoara and An. triannulatus presented nucleotide divergences of 0.35% between An. triannulatus and An. darlingiand of 0.47% between An. darlingi and An. marajoara. This data showed ample interspecific nucleotide variation, though with low nucleotide divergence. The cladogram separated the species of the subgenus Nyssorhynchus and those of the subgenera Cellia and Anopheles, with 98% bootstrap. The region control data did not show strong phylogenetic support, as indicated by the transition/transversion mean rate (0.4643), which is necessary for increasing fragment size and using other more conservative genes for greater inference concerning the phylogeny of the Anopheles species of the subgenus Nyssorhynchus. © Borges-Moroni et al.en
dc.relation.ispartofVolume 5, Número 1, Pags. 6-11pt_BR
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Brazil*
dc.subjectDna, Mitochondrialen
dc.subjectAnopheles Benarrochien
dc.subjectAnopheles Darlingien
dc.subjectAnopheles Marajoaraen
dc.subjectAnopheles Mattogrossensisen
dc.subjectAnopheles Oswaldoien
dc.subjectAnopheles Rangelien
dc.subjectAnopheles Triannulatusen
dc.subjectControlled Studyen
dc.subjectDna Polymorphismen
dc.subjectGene Mutationen
dc.subjectGenetic Distanceen
dc.subjectGenetic Variabilityen
dc.subjectNucleic Acid Base Substitutionen
dc.subjectPolymorphic Locusen
dc.subjectPriority Journalen
dc.subjectSpecies Differenceen
dc.subjectSpecies Identificationen
dc.titleGenetic differentiation in species of Anopheles from the subgenera Nyssorhynchus based on mitochondrial DNAen
dc.publisher.journalOpen Tropical Medicine Journalpt_BR
Appears in Collections:Artigos

Files in This Item:
File Description SizeFormat 
artigo-inpa.pdf336,55 kBAdobe PDFThumbnail

This item is licensed under a Creative Commons License Creative Commons