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https://repositorio.inpa.gov.br/handle/1/16161
Título: | Lipase activity among bacteria isolated from amazonian soils |
Autor: | Willerding, André Luis Oliveira, Luiz Antonio de Moreira, Francisco Wesen Germano, Mariana Gomes Chagas Júnior, A. F. |
Palavras-chave: | 4 Nitrophenylpalmitate Palmitic Acid Derivative Triacylglycerol Lipase Unclassified Drug Bacterial Strain Bacterium Isolation Biotransformation Burkholderia Enzyme Activity Enzyme Assay Enzyme Synthesis Microflora Nonhuman Petri Dish Priority Journal Pseudomonas Qualitative Analysis Soil Microflora Temperature |
Data do documento: | 2011 |
Revista: | Enzyme Research |
É parte de: | Volume 2011, Número 1 |
Abstract: | The objective of this study was to select lipase-producing bacteria collected from different counties of the Amazon region. Of the 440 bacteria strains, 181 were selected for the lipase assay in qualitative tests at Petri dishes, being 75 (41) lipase positive. The enzymatic index was determined during fifteen days at different temperatures (30°, 35°, 40°, and 45°C). The highest lipase activity was observed within 72 hours at 30°C. Twelve bacteria strains presented an index equal to or greater than the standard used like reference, demonstrating the potential of microbial resource. After the bioassay in Petri dishes, the selected bacteria strains were analyzed in quantitative tests on p-nitrophenyl palmitate (p-NPP). A group of the strains was selected for other phases of study with the use in oleaginous substrates of the Amazonian flora, aiming for the application in processes like oil biotransformation. © 2011 André Luis Willerding et al. |
DOI: | 10.4061/2011/720194 |
Aparece nas coleções: | Artigos |
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