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Título: Lipase activity among bacteria isolated from amazonian soils
Autor: Willerding, André Luis
Oliveira, Luiz Antonio de
Moreira, Francisco Wesen
Germano, Mariana Gomes
Chagas Júnior, A. F.
Palavras-chave: 4 Nitrophenylpalmitate
Palmitic Acid Derivative
Triacylglycerol Lipase
Unclassified Drug
Bacterial Strain
Bacterium Isolation
Enzyme Activity
Enzyme Assay
Enzyme Synthesis
Petri Dish
Priority Journal
Qualitative Analysis
Soil Microflora
Data do documento: 2011
Revista: Enzyme Research
Encontra-se em: Volume 2011, Número 1
Abstract: The objective of this study was to select lipase-producing bacteria collected from different counties of the Amazon region. Of the 440 bacteria strains, 181 were selected for the lipase assay in qualitative tests at Petri dishes, being 75 (41) lipase positive. The enzymatic index was determined during fifteen days at different temperatures (30°, 35°, 40°, and 45°C). The highest lipase activity was observed within 72 hours at 30°C. Twelve bacteria strains presented an index equal to or greater than the standard used like reference, demonstrating the potential of microbial resource. After the bioassay in Petri dishes, the selected bacteria strains were analyzed in quantitative tests on p-nitrophenyl palmitate (p-NPP). A group of the strains was selected for other phases of study with the use in oleaginous substrates of the Amazonian flora, aiming for the application in processes like oil biotransformation. © 2011 André Luis Willerding et al.
DOI: 10.4061/2011/720194
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