Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/16211
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dc.contributor.authorOliveira, Sabrina Alberti Nóbrega de-
dc.contributor.authorIbraim, Izabela Coimbra-
dc.contributor.authorTadei, Wanderli Pedro-
dc.contributor.authorRuiz, Jerônimo Conceição-
dc.contributor.authorNahum, Laila Alves-
dc.contributor.authorBrito, Cristiana Ferreira Alves de-
dc.contributor.authorMoreira, Luciano Andrade-
dc.date.accessioned2020-05-31T18:24:28Z-
dc.date.available2020-05-31T18:24:28Z-
dc.date.issued2011-
dc.identifier.urihttps://repositorio.inpa.gov.br/handle/1/16211-
dc.description.abstractBackground: Malaria has a devastating impact on worldwide public health in many tropical areas. Studies on vector immunity are important for the overall understanding of the parasite-vector interaction and for the design of novel strategies to control malaria. A member of the fibrinogen-related protein family, fbn9, has been well studied in Anopheles gambiae and has been shown to be an important component of the mosquito immune system. However, little is known about this gene in neotropical anopheline species. Methods. This article describes the identification and characterization of the fbn9 gene partial sequences from four species of neotropical anopheline primary and secondary vectors: Anopheles darlingi, Anopheles nuneztovari, Anopheles aquasalis, and Anopheles albitarsis (namely Anopheles marajoara). Degenerate primers were designed based on comparative analysis of publicly available Aedes aegypti and An. gambiae gene sequences and used to clone putative homologs in the neotropical species. Sequence comparisons and Bayesian phylogenetic analyses were then performed to better understand the molecular diversity of this gene in evolutionary distant anopheline species, belonging to different subgenera. Results: Comparisons of the fbn9 gene sequences of the neotropical anophelines and their homologs in the An. gambiae complex (Gambiae complex) showed high conservation at the nucleotide and amino acid levels, although some sites show significant differentiation (non-synonymous substitutions). Furthermore, phylogenetic analysis of fbn9 nucleotide sequences showed that neotropical anophelines and African mosquitoes form two well-supported clades, mirroring their separation into two different subgenera. Conclusions: The present work adds new insights into the conserved role of fbn9 in insect immunity in a broader range of anopheline species and reinforces the possibility of manipulating mosquito immunity to design novel pathogen control strategies. © 2011 Oliveira et al; licensee BioMed Central Ltd.en
dc.language.isoenpt_BR
dc.relation.ispartofVolume 10pt_BR
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/br/*
dc.subjectAmino Aciden
dc.subjectFibrinogen Related Protein 9en
dc.subjectInsect Proteinsen
dc.subjectNucleotideen
dc.subjectUnclassified Drugen
dc.subjectFibrinogenen
dc.subjectImmunoglobulinen
dc.subjectAedes Aegyptien
dc.subjectAmino Acid Substitutionen
dc.subjectAnophelesen
dc.subjectAnopheles Albitarsisen
dc.subjectAnopheles Aquasalisen
dc.subjectAnopheles Darlingien
dc.subjectAnopheles Marajoaraen
dc.subjectAnopheles Merusen
dc.subjectAnopheles Nuneztovarien
dc.subjectAnopheles Quadriannulatusen
dc.subjectBayes Theoremen
dc.subjectCladisticsen
dc.subjectComparative Studyen
dc.subjectControlled Studyen
dc.subjectDisease Carrieren
dc.subjectEvolutionary Homologyen
dc.subjectGene Identificationen
dc.subjectGene Sequenceen
dc.subjectGenetic Variabilityen
dc.subjectGenotypeen
dc.subjectCloning, Molecularen
dc.subjectNeotropicsen
dc.subjectNonhumanen
dc.subjectNucleotide Sequenceen
dc.subjectPhylogenyen
dc.subjectSequence Homologyen
dc.subjectPolymorphism, Single Nucleotideen
dc.subjectAmino Acid Sequenceen
dc.subjectAnimalsen
dc.subjectClassificationen
dc.subjectGeneen
dc.subjectGeneticsen
dc.subjectImmunologyen
dc.subjectMalariaen
dc.subjectEvolution, Molecularen
dc.subjectParasitologyen
dc.subjectSequence Analysisen
dc.subjectAmino Acid Sequenceen
dc.subjectAnimalen
dc.subjectAnophelesen
dc.subjectBase Sequenceen
dc.subjectCloning, Molecularen
dc.subjectEvolution, Molecularen
dc.subjectFibrinogenen
dc.subjectGenes, Insecten
dc.subjectImmunoglobulinsen
dc.subjectInsect Vectorsen
dc.subjectMalariaen
dc.subjectPhylogenyen
dc.subjectSequence Analysisen
dc.titleIdentification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoesen
dc.typeArtigopt_BR
dc.identifier.doi10.1186/1475-2875-10-21-
dc.publisher.journalMalaria Journalpt_BR
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