Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/16248
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSantos, Mayara S.-
dc.contributor.authorSouza, Érica Simplício de-
dc.contributor.authorJunior, R. M.S.-
dc.contributor.authorTalhari, Sine?io-
dc.contributor.authorSouza, João Vicente Braga de-
dc.date.accessioned2020-06-02T15:09:53Z-
dc.date.available2020-06-02T15:09:53Z-
dc.date.issued2010-
dc.identifier.urihttps://repositorio.inpa.gov.br/handle/1/16248-
dc.description.abstractPrompt and specific identification of fungemia agents is important in order to define clinical treatment. However, in most cases conventional culture identification can be considered to be time-consuming and not without errors. The aim of the present study was to identify the following fungemia agents: Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata, Cryptococcus neoformans, Cryptococcus gattii, and Histoplasma capsulatum using the polymerase chain reaction and restriction fragment length polymorphism analysis (PCR/RFLP). More specifically: a) to evaluate 3 different amplification regions, b) to investigate 3 different restriction enzymes, and c) to use the best PCR/RFLP procedure to indentify 60 fungemia agents from a culture collection. All 3 pairs of primers (ITS1/ITS4, NL4/ITS5 and Primer1/Primer2) were able to amplify DNA from the reference strains. However, the size of these PCR products did not permit the identification of all the species studied. Three restriction enzymes were used to digest the PCR products: HaeIII, Ddel and Bfal. Among the combinations of pairs of primers and restriction enzymes, only one (primer pair NL4/ITS5 and restriction enzyme Ddel) produced a specific RFLP pattern for each microorganism studied. Sixty cultures of fungemia agents (selected from the culture collection of Fundação de Medicina Tropical do Amazonas - FMTAM) were correctly identified by PCR/RFLP using the prime pair NL4/ITS5 and Ddel. We conclude that the method proved to be both simple and reproducible, and may offer potential advantages over phenotyping methods.en
dc.language.isoenpt_BR
dc.relation.ispartofVolume 43, Número 8, Pags. 712-716pt_BR
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/br/*
dc.subjectPrimer Dnaen
dc.subjectRestriction Endonucleaseen
dc.subjectCandida Albicansen
dc.subjectCandida Glabrataen
dc.subjectCandida Parapsilosisen
dc.subjectCandida Tropicalisen
dc.subjectCryptococcus Gattiien
dc.subjectCryptococcus Neoformansen
dc.subjectFungal Detectionen
dc.subjectFungal Strainen
dc.subjectFungemiaen
dc.subjectFungusen
dc.subjectFungus Cultureen
dc.subjectGene Amplificationen
dc.subjectHistoplasma Capsulatumen
dc.subjectHumanen
dc.subjectNonhumanen
dc.subjectPolymerase Chain Reactionen
dc.subjectPolymorphism, Restriction Fragment Lengthen
dc.subjectAids-related Opportunistic Infectionsen
dc.subjectCandidaen
dc.subjectCryptococcusen
dc.subjectDna, Fungalen
dc.subjectDna, Ribosomal Spaceren
dc.subjectFungemiaen
dc.subjectHistoplasmaen
dc.subjectHumansen
dc.subjectPolymerase Chain Reactionen
dc.subjectPolymorphism, Restriction Fragment Lengthen
dc.subjectAjellomyces Capsulatusen
dc.subjectCandida Albicansen
dc.subjectCandida Glabrataen
dc.subjectCandida Parapsilosisen
dc.subjectCandida Tropicalisen
dc.subjectCryptococcus Gattiien
dc.subjectFilobasidiella Neoformansen
dc.titleIdentification of fungemia agents using the polymerase chain reaction and restriction fragment length polymorphism analysisen
dc.typeArtigopt_BR
dc.identifier.doi10.1590/S0100-879X2010007500065-
dc.publisher.journalBrazilian Journal of Medical and Biological Researchpt_BR
Appears in Collections:Artigos

Files in This Item:
File Description SizeFormat 
artigo-inpa.pdf843,22 kBAdobe PDFThumbnail
View/Open


This item is licensed under a Creative Commons License Creative Commons