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Title: Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display
Authors: Siquieroli, Ana Carolina Silva
Vieira, Carlos Ueira
Carvalho-Zelse, Gislene A.
Goulart, L. R.
Kerr, Warwick Estevam
Bonetti, Ana Maria
Keywords: Rna, Messenger
Abdominal Ganglion
Cell Count
Controlled Study
Gene Expression Profiling
Genetic Transcription
Genetic Variability
Insect Caste
Melipona Scutellaris
Pupa (life Cycle Stage)
Queen (insect)
Reverse Transcription Polymerase Chain Reaction
Rna Analysis
Species Comparison
Stingless Bee
Worker (insect)
Melipona Scutellaris
Issue Date: 2009
metadata.dc.publisher.journal: Biological Research
metadata.dc.relation.ispartof: Volume 42, Número 1, Pags. 107-110
Abstract: In colonies of Melipona scutellaris Latreille, 1811 workers can be found with four ganglion nerve cells, a morphological characteristic of the queen. It is hypothesized that these workers, called intercastes, or phenocopies, are phenotypically-like workers, but genotypically identical to queens due to this specific trait. Workers with the same number of ganglion as queens seem to be intercastes between queens and workers. Our objective was to analyze the mRNA profiles of workers, queens, and intercastes of M. scutellaris through DDRT-PCR. Three hundred (300) pupae with white eyes were collected and externally identified according to the number of abdominal nerve ganglions: workers (5 ganglions), queens (4 ganglions) and intercastes (4 ganglions). The analysis identified differentially expressed transcripts that were present only in workers, but absent in intercastes and queens, confirming the hypothesis, by demonstrating the environmental effect on the queen genotype that generated phenotype-like workers.
metadata.dc.identifier.doi: 10.4067/S0716-97602009000100011
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