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Title: Genetic polymorphism and molecular epidemiology of Leishmania (Viannia) braziliensis from different hosts and geographic areas in Brazil
Authors: Cupolillo, Elisa
Brahim, Lúcia Regina
Toaldo, Cristiane Bentin
Paes de Oliveira-Neto, Manoel
Brito, Maria Edileuza Felinto de
Falqueto, Aloi?io
Naiff, Maricleide de Farias
Grimaldi Júnior, Gabriel F.
Keywords: Controlled Study
Epidemiological Data
Genetic Epidemiology
Polymorphism, Genetic
Geographic Distribution
Geographic Pathology
Host Parasite Interaction
Leishmania Braziliensis
Microbial Diversity
Parasite Vector
Polymerase Chain Reaction
Priority Journal
Polymorphism, Restriction Fragment Length
Species Endemicity
Strain Identification
Dna, Protozoan
Dna, Ribosomal Spacer
Epidemiology, Molecular
Genes, Rrna
Host-parasite Relations
Leishmania Braziliensis
Leishmaniasis, Cutaneous
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Leishmania Braziliensis
Issue Date: 2003
metadata.dc.publisher.journal: Journal of Clinical Microbiology
metadata.dc.relation.ispartof: Volume 41, Número 7, Pags. 3126-3132
Abstract: Numerical zymotaxonomy and variability of the internal transcribed spacers (ITS) between the small and large subunits of the rRNA genes were used to examine strain variation and relationships in natural populations of Leishmania (Viannia) braziliensis. A total of 101 strains from distinct hosts and Brazilian geographic regions were assigned to 15 zymodemes clustered in two major genetic groups. The great number of isolates (48.5%) placed in zymodeme IOC/Z-27 were collected on the Atlantic coast. The high molecular diversity found in populations in the Amazon Basin was related to the great number of sandfly vector(s) in that region. The results of the restriction fragment length polymorphism analysis of the ITS depicted considerable intraspecific variation. Genotypic groups A, B, and C contained 39, 40, and 22 isolates, which were divided into 16, 10, and 15 genotypes, respectively. The genetic polymorphism observed demonstrates the degree of diversity of L. (V.) braziliensis strains from different regions where they are endemic. The results reinforce the clonal theory for Leishmania parasites showing the genetic diversity of this pathogen and an association of L. (V.) braziliensis genotypes with specific transmission cycles, probably reflecting an adaptation of different clones to the vector species involved.
metadata.dc.identifier.doi: 10.1128/JCM.41.7.3126-3132.2003
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