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Title: Ontogenetic patterns and genetic variation in Anopheles (Anopheles) intermedius Chagas, 1908 and Anopheles (Anopheles) mattogrossensis Lutz & Neiva, 1911 (Diptera: Culicidae) in the Brazilian amazon.
Authors: Rodriguez, Gloria Alicia Diaz
Santos, Joselita Maria Mendes dos
Maia, Juracy de Freitas
Keywords: Cytosol Aminopeptidase
Glycerol 3 Phosphate Dehydrogenase
Gene Expression Regulation
Genetic Variability
Gene Expression Regulation, Enzymologic
Glycerolphosphate Dehydrogenase
Leucyl Aminopeptidase
Variation (genetics)
Issue Date: 2000
metadata.dc.publisher.journal: Revista Brasileira de Biologia
metadata.dc.relation.ispartof: Volume 60, Número 2, Pags. 341-351
Abstract: Changes in the expression of genes were observed during development in populations of Anopheles (Anopheles) intermedius and Anopheles (Anopheles) mattogrossensis. Esterase showed seven zones of activity: EST1 was present in all developmental stages of both species; EST2 was observed only in larvae of A. intermedius and larvae and pupae of A. mattogrossensis, with greater activity in pupae; EST3 and EST5 were present in all developmental stages, with greater intensity in larvae; EST4 and EST6 showed weak activity in larvae of A. mattogrossensis and was not found in A. intermedius. Leucine aminopeptidase showed four zones of activity, of which LAP1 and LAP2 were found in all stages of A. intermedius, with highest activity in larvae, and in larvae only of A. mattogrossensis. LAP3 was detected in all stages of A. mattogrossensis and in larvae only of A. intermedius. LAP4 was detected only in larvae and pupae of A. mattogrossensis, with greater intensity in pupae. alpha-Glycerophosphate dehydrogenase showed a single zone of activity, detected in older fourth-instar larvae and becoming more intense from the pupal stage onwards.
metadata.dc.identifier.doi: 10.1590/S0034-71082000000200020
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