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Title: Cryptococcus gattii VGII isolated from native forest and river in Northern Brazil
Authors: Bentes, Amaury dos Santos
Wanke, Bodo
dos Santos Lazéra, Márcia
Freire, Ana Karla Lima L.
Silva Júnior, Roberto Moreira da
Rocha, Diego Fernando Silva
Pinheiro, Silviane Bezerra
Zelski, Steven Edward
Matsuura, Ani Beatriz Jackisch
Rocha, Liliane Coelho da
Souza, Érica Simplício de
Souza, João Vicente Braga de
Keywords: Dna, Fungal
Cryptococcus Gattii
Isolation And Purification
Microbiological Examination
Molecular Typing
Cryptococcus Gattii
Dna, Fungal
Molecular Typing
Mycological Typing Techniques
Soil Microbiology
Issue Date: 2019
metadata.dc.publisher.journal: Brazilian Journal of Microbiology
metadata.dc.relation.ispartof: Volume 50, Número 2, Pags. 495-500
Abstract: Background: Cryptococcosis is a global invasive mycosis associated with significant morbidity and mortality. In the northern region of Brazil, this disease is caused by Cryptococcus neoformans genotype VNI and Cryptococcus gattii genotype VGII. However, few environmental studies have been conducted in this large tropical area. Aims: This study was performed to isolate, genotype, and determine the frequency of cryptococcal agents in environmental samples near Manaus, Amazonas, Brazil. Methods: A total of 970 environmental samples (290 from soil, 290 from decaying plants, 5 from insects, 280 from the Negro river, and 105 from small streams within the city of Manaus) were collected and plated on Niger seed agar. In addition, 20 sub-cultures obtained from each positive sample were analyzed by PCR-RFLP (URA5) and PCR for genotyping and determination of mating type. Results: Six samples were positive for isolates from the C. gattii species complex. Of those, three samples were from Adolpho Ducke Forest Reserve and three were from the Negro river. All isolates were C. gattii genotype VGII (mating type MATα). Conclusion: Genotype VGII proved to be the most important genotype found in the environmental samples. The genotype VGII has been described as one of the most virulent and less susceptible to antifungals and responsible for important outbreaks. This is the first study to demonstrate isolation of C. gattii (VGII) from the Negro river. © 2019, Sociedade Brasileira de Microbiologia.
metadata.dc.identifier.doi: 10.1007/s42770-019-00066-6
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