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Title: Characterization of spermatogonial cells and niche in the scorpion mud turtle (Kinosternon scorpioides)
Authors: Costa, Guilherme M.J.
Sousa, Alana Lislea
Figueiredo, Andre´ Felipe Almeida
Lacerda, Samyra Maria Santos N.Nassif
França, Luiz Renato de
Keywords: Colony Stimulating Factor 1 Receptor
Colony Stimulating Factor Receptor
Gdnf Family Receptor Alpha 1
Glial Cell Line Derived Neurotrophic Factor Receptor
Unclassified Drug
Biological Marker
Glial Cell Line Derived Neurotrophic Factor Receptor
Granulocyte Macrophage Colony Stimulating Factor Receptor
Animals Cell
Animals Experiment
Animals Tissue
Cell Culture
Cell Size
Cell Structure
Cellular Distribution
Controlled Study
Genetic Conservation
Kinosternon Scorpioides
Priority Journal
Protein Expression
Stem Cell Niche
Cell Shape
South America
Stem Cell
Cell Shape
Cell Size
Glial Cell Line-derived Neurotrophic Factor Receptors
Receptors, Granulocyte-macrophage Colony-stimulating Factor
South America
Stem Cell Niche
Stem Cells
Issue Date: 2019
metadata.dc.publisher.journal: General and Comparative Endocrinology
metadata.dc.relation.ispartof: Volume 273, Pags. 163-171
Abstract: Undifferentiated spermatogonia (A und ) or spermatogonial stem cells (SSCs) are committed to the establishment and maintenance of spermatogenesis and fertility throughout a male's life and are located in a highly specialized microenvironment called niche that regulates their fate. Although several studies have been developed on SSCs in mammalian testis, little is known about other vertebrate classes. The present study is the first to perform a more detailed investigation on the spermatogonial cells and their niche in a reptilian species. Thus, we characterized A und /SSCs and evaluated the existence of SSCs niche in the Kinosternon scorpioides, a freshwater turtle found from Mexico to northern and central South America. Our results showed that, in this species, A und /SSCs exhibited a nuclear morphological pattern similar to those described for other mammalian species already investigated. However, in comparison to other spermatogonial cell types, A und /SSCs presented the largest nuclear volume in this turtle. Similar to some mammalian and fish species investigated, both GFRA1 and CSF1 receptors were expressed in A und /SSCs in K. scorpioides. Also, as K. scorpioides A und /SSCs were preferentially located near blood vessels, it can be suggested that this niche characteristic is a well conserved feature during evolution. Besides being valuable for comparative reproductive biology, our findings represent an important step towards the understanding of SSCs biology and the development of valuable systems/tools for SSCs culture and cryopreservation in turtles. Moreover, we expect that the above-mentioned results will be useful for reproductive biotechnologies as well as for governmental programs aiming at reptilian species conservation. © 2018 Elsevier Inc.
metadata.dc.identifier.doi: 10.1016/j.ygcen.2018.06.019
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