Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/16894
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dc.contributor.authorRamos, Márcio Viana-
dc.contributor.authorBrito, Daniel-
dc.contributor.authorFreitas, Cléverson Diniz Teixeira de-
dc.contributor.authorGonçalves, José Francisco de Carvalho-
dc.contributor.authorPorfírio, Camila T.M.N.-
dc.contributor.authorLobo, Marina Duarte Pinto-
dc.contributor.authorMonteiro, Cristina A.-
dc.contributor.authorSouza, Luis Augusto Gomes-
dc.contributor.authorFernandes, Andréia Varmes-
dc.date.accessioned2020-06-15T21:37:05Z-
dc.date.available2020-06-15T21:37:05Z-
dc.date.issued2018-
dc.identifier.urihttps://repositorio.inpa.gov.br/handle/1/16894-
dc.description.abstractMain conclusion: Seeds of native species from the rain forest (Amazon) are source of chitinases and their protein extracts exhibited strong and broad antifungal activity. Numerous plant species native to the Amazon have not yet been chemically studied. Studies of seeds are scarcer, since adversities in accessing study areas and seasonality pose constant hurdles to systematic research. In this study, proteins were extracted from seeds belonging to endemic Amazon species and were investigated for the first time. Proteolytic activity, peptidase inhibitors, and chitinases were identified, but chitinolytic activity predominated. Four proteins were purified through chromatography and identified as lectin and chitinases by MS/MS analyses. The proteins were examined for inhibition of a phytopathogen (Fusarium oxysporum). Analyses by fluorescence microscopy suggested binding of propidium iodide to DNA of fungal spores, revealing that spore integrity was lost when accessed by the proteins. Further structural and functional analyses of defensive proteins belonging to species facing highly complex ecosystems such as Amazonia should be conducted, since these could provide new insights into specificity and synergism involving defense proteins of plants submitted to a very complex ecosystem. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.en
dc.language.isoenpt_BR
dc.relation.ispartofVolume 248, Número 1, Pags. 197-209pt_BR
dc.rightsRestrito*
dc.subjectAntifungal Agenten
dc.subjectChitinaseen
dc.subjectLectinen
dc.subjectPlant Proteinen
dc.subjectChemistryen
dc.subjectDrug Effecten
dc.subjectFabaceaeen
dc.subjectMicroscopy, Fluorescenceen
dc.subjectFungus Sporeen
dc.subjectFusariumen
dc.subjectIsolation And Purificationen
dc.subjectMass Spectrometryen
dc.subjectSeed Planten
dc.subjectElectrophoresis, Polyacrylamide Gelen
dc.subjectProteomicsen
dc.subjectRainforesten
dc.subjectAntifungal Agentsen
dc.subjectChitinasesen
dc.subjectElectrophoresis, Polyacrylamide Gelen
dc.subjectFabaceaeen
dc.subjectFusariumen
dc.subjectLectinsen
dc.subjectMass Spectrometryen
dc.subjectMicroscopy, Fluorescenceen
dc.subjectPlant Proteinsen
dc.subjectProteomicsen
dc.subjectRainforesten
dc.subjectSeedsen
dc.subjectSpores, Fungalen
dc.titleProteomic identification and purification of seed proteins from native Amazonian species displaying antifungal activityen
dc.typeArtigopt_BR
dc.identifier.doi10.1007/s00425-018-2893-y-
dc.publisher.journalPlantapt_BR
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