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Campo DC | Valor | Idioma |
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dc.contributor.author | Costa, Guilherme M.J. | - |
dc.contributor.author | Avelar, Gleide Fernandes | - |
dc.contributor.author | Lacerda, Samyra Maria Santos N.Nassif | - |
dc.contributor.author | Figueiredo, Andre´ Felipe Almeida | - |
dc.contributor.author | Tavares, Amanda O. | - |
dc.contributor.author | Rezende-Neto, José V. | - |
dc.contributor.author | Martins, Felipe G.P. | - |
dc.contributor.author | França, Luiz Renato de | - |
dc.date.accessioned | 2020-06-15T21:38:01Z | - |
dc.date.available | 2020-06-15T21:38:01Z | - |
dc.date.issued | 2017 | - |
dc.identifier.uri | https://repositorio.inpa.gov.br/handle/1/17005 | - |
dc.description.abstract | The establishment of proper conditions for spermatogonial stem cells (SSCs) cryopreservation and storage represents an important biotechnological approach for the preservation of the genetic stock of valuable animals. This study demonstrates the effects of different cryopreservation protocols on the survival rates and phenotypic expression of SSCs in horses. The cells were enzymatically isolated from testes of eight adult horses. After enrichment and characterization of germ cells in the suspension, the feasibility of several cryopreservation protocols were evaluated. Three different cryomedia compositions, associated with three different methods of freezing (vitrification, slow-freezing and fast-freezing) were evaluated. Based on the rates of viable SSCs found before and after thawing, as well as the number of recovered cells after cryopreservation, the best results were obtained utilizing the DMSO-based cryomedia associated with the slow-freezing method. In addition, when isolated cells were cultured in vitro, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and immunofluorescence analysis indicated that the cryopreserved cells were as metabolically active as the fresh cells and were also expressing typical SSCs proteins (VASA, NANOS2 and GFRA1). Therefore, our results indicate that equine SSCs can be cryopreserved without impairment of structure, function, or colony-forming abilities. © 2017, Springer-Verlag GmbH Germany. | en |
dc.language.iso | en | pt_BR |
dc.relation.ispartof | Volume 370, Número 3, Pags. 489-500 | pt_BR |
dc.rights | Restrito | * |
dc.subject | Dead Box4 Rna Helicase Expressed In The Germ Cells Protein | en |
dc.subject | Dimethyl Sulfoxide | en |
dc.subject | Glial Cell Line Derived Neurotrophic Factor Receptor | en |
dc.subject | Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 | en |
dc.subject | Nanos C2hc Type Zinc Finger 2 Protein | en |
dc.subject | Rna Helicase | en |
dc.subject | Unclassified Drug | en |
dc.subject | Zinc Finger Protein | en |
dc.subject | Cryoprotective Agent | en |
dc.subject | Dimethyl Sulfoxide | en |
dc.subject | Adult | en |
dc.subject | Animals Cell | en |
dc.subject | Animals Genetics | en |
dc.subject | Biotechnology | en |
dc.subject | Cell Assay | en |
dc.subject | Cell Count | en |
dc.subject | Cell Culture | en |
dc.subject | Cell Function | en |
dc.subject | Cell Isolation | en |
dc.subject | Cell Structure | en |
dc.subject | Cell Survival | en |
dc.subject | Cell Viability | en |
dc.subject | Controlled Study | en |
dc.subject | Cryopreservation | en |
dc.subject | Fast Freezing Method | en |
dc.subject | Feasibility Study | en |
dc.subject | Genetic Gain | en |
dc.subject | Genetic Resources | en |
dc.subject | Genome | en |
dc.subject | Horse | en |
dc.subject | Immunofluorescence Test | en |
dc.subject | In Vitro Study | en |
dc.subject | Intermethod Comparison | en |
dc.subject | Nonhuman | en |
dc.subject | Phenotype | en |
dc.subject | Priority Journal | en |
dc.subject | Protein Expression | en |
dc.subject | Slow Freezing Method | en |
dc.subject | Spermatogonium | en |
dc.subject | Survival Rate | en |
dc.subject | Vitrification | en |
dc.subject | Adult Stem Cell | en |
dc.subject | Animals | en |
dc.subject | Cryopreservation | en |
dc.subject | Cytology | en |
dc.subject | Male | en |
dc.subject | Parenchyma | en |
dc.subject | Procedures | en |
dc.subject | Sperm Preservation | en |
dc.subject | Spermatogonium | en |
dc.subject | Testis | en |
dc.subject | Vitrification | en |
dc.subject | Adult Germline Stem Cells | en |
dc.subject | Animal | en |
dc.subject | Cell Survival | en |
dc.subject | Cryopreservation | en |
dc.subject | Cryoprotective Agents | en |
dc.subject | Dimethyl Sulfoxide | en |
dc.subject | Horses | en |
dc.subject | Male | en |
dc.subject | Parenchymal Tissue | en |
dc.subject | Semen Preservation | en |
dc.subject | Spermatogonia | en |
dc.subject | Testis | en |
dc.subject | Vitrification | en |
dc.title | Horse spermatogonial stem cell cryopreservation: feasible protocols and potential biotechnological applications | en |
dc.type | Artigo | pt_BR |
dc.identifier.doi | 10.1007/s00441-017-2673-1 | - |
dc.publisher.journal | Cell and Tissue Research | pt_BR |
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