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Title: Chromosomal Mapping of Repetitive Sequences (Rex3, Rex6, and rDNA Genes) in Hybrids Between Colossoma macropomum (Cuvier, 1818) and Piaractus mesopotamicus (Holmberg, 1887)
Authors: Braga Ribeiro, Leila
Moraes-Neto, Américo
Artoni, Roberto Ferreira
Matoso, D. A.
Feldberg, Eliana
Keywords: Fish Protein
Ribosome Dna
Chromosomal Mapping
Gene Expression Regulation
Nucleotide Repeat
Chromosome Mapping
Ribosomal Dna
Fish Proteins
Gene Expression Regulation
Hybridization, Genetic
Repetitive Sequences, Nucleic Acid
Issue Date: 2017
metadata.dc.publisher.journal: Zebrafish
metadata.dc.relation.ispartof: Volume 14, Número 2, Pags. 155-160
Abstract: Some species of Characiformes are known for their high economic value, such as Colossoma macropomum and Piaractus mesopotamicus, and are used in aquaculture programs to generate hybrid tambacu (interbreeding of C. macropomum females and P. mesopotamicus males). The present work aimed to investigate the location of the Rex3 and Rex6 transposable elements in the hybrid and in the species, in addition to checking the genomic organization of the 18S and 5S rDNA in tambacu. The diploid number found for the hybrid was equal to 54 chromosomes, with heterochromatic blocks distributed mainly in the centromeric portions. The chromosomal location of the mobile elements Rex3 and Rex6 in C. macropomum, P. mesopotamicus, and in the hybrid between these species enabled knowledge expansion and the generation of data on such mobile elements. In addition, the location of such elements is not related to the distribution of ribosomal DNA sites. The mapping of the 18S rDNA was shown to be effective in cytogenetic identification of the hybrid tambacu, allowing for differentiation from the parent species and from the hybrid between C. macropomum and the other species from Piaractus (P. brachypomus).
metadata.dc.identifier.doi: 10.1089/zeb.2016.1378
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