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Title: Genomic Organization under Different Environmental Conditions: Hoplosternum Littorale as a Model
Authors: Silva, Francijara Araújo da
Schneider, Carlos Henrique
Feldberg, Eliana
Baccaro, Fabricio Beggiato
Carvalho, Natália Dayane Moura
Gross, Maria Claudia
Keywords: Ribosome Dna
Rna 18s
Rna 5s
Chromosomal Mapping
Epigenesis, Genetic
Chromosome Mapping
Ribosomal Dna
Epigenesis, Genetic
Rna, Ribosomal, 18s
Rna, Ribosomal, 5s
Issue Date: 2016
metadata.dc.publisher.journal: Zebrafish
metadata.dc.relation.ispartof: Volume 13, Número 3, Pags. 197-208
Abstract: The Amazon has abundant rivers, streams, and floodplains in both polluted and nonpolluted environments, which show great adaptability. Thus, the goal of this study was to map repetitive DNA sequences in both mitotic chromosomes and erythrocyte micronuclei of tamoatás from polluted and nonpolluted environments and to assess the possible genotoxic effects of these environments. Individuals were collected in Manaus, Amazonas (AM), and submitted to classical and molecular cytogenetic techniques, as well as to a blood micronucleus test. Diploid number equal to 60 chromosomes are present in all individuals, with 18S ribosomal DNA sites present in one chromosome pair and no interstitial telomeric sites on chromosomes. The micronucleus test showed no significant differences in pairwise comparisons between environments or collection sites, but the Rex3 retroelement was dispersed on the chromosomes of individuals from unpolluted environments and compartmentalized in individuals from polluted environments. Divergent numbers of 5S rDNA sites are present in individuals from unpolluted and polluted environments. The mapping of repetitive sequences revealed that micronuclei have different compositions both intra- and interindividually that suggests different regions are lost in the formation of micronuclei, and no single fragile region undergoes breaks, although repetitive DNA elements are involved in this process. © Copyright 2016, Mary Ann Liebert, Inc. 2016.
metadata.dc.identifier.doi: 10.1089/zeb.2015.1237
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