Use este identificador para citar ou linkar para este item: https://repositorio.inpa.gov.br/handle/1/17328
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dc.contributor.authorSilva, Thaís Pereira da-
dc.contributor.authorMoura, Valéria Mourão de-
dc.contributor.authorSouza, Maria Carolina Scheffer de-
dc.contributor.authorSantos, Vanessa Neves Carvalho-
dc.contributor.authorSilva, Kiriaki Nurit M.Moreira da-
dc.contributor.authorMendes, Mara Giselle Guimarães-
dc.contributor.authorNunez, C. V.-
dc.contributor.authorAlmeida, Patrícia Danielle Oliveira de-
dc.contributor.authorLima, Emerson Silva-
dc.contributor.authorMourão, Rosa Helena Veras-
dc.contributor.authorSantos, Maria Cristina dos-
dc.date.accessioned2020-06-15T21:41:19Z-
dc.date.available2020-06-15T21:41:19Z-
dc.date.issued2016-
dc.identifier.urihttps://repositorio.inpa.gov.br/handle/1/17328-
dc.description.abstractEthnopharmacological relevance The plant species Connarus favosus is used in folk medicine in the west of Pará state, Brazil, to treat snakebites. Aim of the study To investigate the potential of the aqueous extract of Connarus favosus (AECf) to inhibit hemorrhagic and phospholipase A2 activities induced by Bothrops atrox venom (BaV) and to determine the antioxidant and antimicrobial potentials of the extract. Materials and methods AECf was analyzed phytochemically for phenolics (condensed tannins and hydrolyzable tannins) by colorimetry. Antioxidant activity was evaluated by quantitative assays using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Fe3+/phenanthroline. Antimicrobial activity was evaluated by the minimal inhibitory concentration test, and cytotoxicity was evaluated using human fibroblast cells (MRC-5). Inhibition of BaV-induced hemorrhagic activity was assessed after oral administration of the extract using pre-treatment, post-treatment and combined (BA plus AECf) treatment protocols. Inhibition of indirect hemolysis caused by phospholipase A2 (PLA2) was investigated in vitro. Interaction between AECf and BaV was investigated by SDS-PAGE electrophoresis, Western blot (Wb) and zymography. Results The phytochemical profile of AECf revealed ten secondary metabolite classes, and colorimetry showed high total phenolic and total (condensed and hydrolyzable) tannin content. AECf exhibited high antioxidant and antimicrobial potentials. The IC50 for the cytotoxic effect was 51.91 (46.86-57.50) μg/mL. Inhibition of BaV-induced hemorrhagic activity was significant in all the protocols, and inhibition of PLA2 activity was significant with the two highest concentrations. The BaV/AECf mixture produced the same bands as BaV by itself in SDS-PAGE and Wb although the bands were much fainter. Zymography confirmed the proteolytic activity of BaV, but when the venom was pre-incubated with AECf this activity was blocked. Conclusion AECf was effective in reducing BaV-induced hemorrhagic activity when administered by the same route as that used in folk medicine and exhibited antioxidant and antimicrobial potentials. © 2016 Elsevier Ireland Ltd. All rights reserved.en
dc.language.isoenpt_BR
dc.relation.ispartofVolume 183, Pags. 166-175pt_BR
dc.rightsRestrito*
dc.subjectAntibiotic Agenten
dc.subjectAntioxidanten
dc.subjectBatroxobinen
dc.subjectConnarus Favosus Extracten
dc.subjectPhenanthrolineen
dc.subjectPhospholipase A2en
dc.subjectPlant Extracten
dc.subjectTannin Derivativeen
dc.subjectUnclassified Drugen
dc.subjectAntiinfective Agenten
dc.subjectAntioxidanten
dc.subjectPhospholipase A2en
dc.subjectPlant Extracten
dc.subjectPlant Medicinal Producten
dc.subjectSnake Venomen
dc.subjectTannin Derivativeen
dc.subjectVenom Antiserumen
dc.subjectAnimals Experimenten
dc.subjectAnimals Modelen
dc.subjectAntibacterial Activityen
dc.subjectAntioxidant Activityen
dc.subjectBleedingen
dc.subjectColorimetryen
dc.subjectControlled Studyen
dc.subjectDpph Radical Scavenging Assayen
dc.subjectDrug Cytotoxicityen
dc.subjectEnvenomationen
dc.subjectEnzyme Activityen
dc.subjectFemaleen
dc.subjectFibroblasten
dc.subjectHemolysisen
dc.subjectHumanen
dc.subjectHuman Cellen
dc.subjectIc 50en
dc.subjectIn Vitro Studyen
dc.subjectMaleen
dc.subjectMinimum Inhibitory Concentrationen
dc.subjectMouseen
dc.subjectNonhumanen
dc.subjectPhytochemistryen
dc.subjectElectrophoresis, Polyacrylamide Gelen
dc.subjectProtein Degradationen
dc.subjectQuantitative Assayen
dc.subjectWestern Blottingen
dc.subjectZymographyen
dc.subjectAnimalsen
dc.subjectBothropsen
dc.subjectChemistryen
dc.subjectConnaraceaeen
dc.subjectHemorrhageen
dc.subjectMetabolismen
dc.subjectProceduresen
dc.subjectSnake Bitesen
dc.subjectMedicine, Traditionalen
dc.subjectAnimalen
dc.subjectAnti-bacterial Agentsen
dc.subjectAntioxidantsen
dc.subjectAntiveninsen
dc.subjectBothropsen
dc.subjectConnaraceaeen
dc.subjectCrotalid Venomsen
dc.subjectFemaleen
dc.subjectHemorrhageen
dc.subjectMaleen
dc.subjectMedicine, Traditionalen
dc.subjectMiceen
dc.subjectPhospholipases A2en
dc.subjectPhytochemicalsen
dc.subjectPlant Extractsen
dc.subjectSnake Bitesen
dc.subjectTanninsen
dc.titleConnarus favosus Planch.: An inhibitor of the hemorrhagic activity of Bothrops atrox venom and a potential antioxidant and antibacterial agenten
dc.typeArtigopt_BR
dc.identifier.doi10.1016/j.jep.2016.02.043-
dc.publisher.journalJournal of Ethnopharmacologypt_BR
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