Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/17372
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dc.contributor.authorBridi, Letícia C.-
dc.contributor.authorRafael, Míriam Silva-
dc.date.accessioned2020-06-15T21:41:54Z-
dc.date.available2020-06-15T21:41:54Z-
dc.date.issued2016-
dc.identifier.urihttps://repositorio.inpa.gov.br/handle/1/17372-
dc.description.abstractAnopheles darlingi is the main malaria vector in humans in South America. In the Amazon basin, it lives along the banks of rivers and lakes, which responds to the annual hydrological cycle (dry season and rainy season). In these breeding sites, the larvae of this mosquito feed on decomposing organic and microorganisms, which can be pathogenic and trigger the activation of innate immune system pathways, such as proteins Gram-negative binding protein (GNBP). Such environmental changes affect the occurrence of polymorphic inversions especially at the heterozygote frequency, which confer adaptative advantage compared to homozygous inversions. We mapped the GNBP probe to the An. darlingi 2Rd inversion by fluorescent in situ hybridization (FISH), which was a good indicator of the GNBP immune response related to the chromosomal polymorphic inversions and adaptative evolution. To better understand the evolutionary relations and time of divergence of the GNBP of An. darlingi, we compared it with nine other mosquito GNBPs. The results of the phylogenetic analysis of the GNBP sequence between the species of mosquitoes demonstrated three clades. Clade I and II included the GNBPB5 sequence, and clade III the sequence of GNBPB1. Most of these sequences of GNBP analyzed were homologous with that of subfamily B, including that of An. gambiae (87 %), therefore suggesting that GNBP of An. darling belongs to subfamily B. This work helps us understand the role of inversion polymorphism in evolution of An. darlingi. © 2016, Springer International Publishing Switzerland.en
dc.language.isoenpt_BR
dc.relation.ispartofVolume 144, Número 1, Pags. 99-106pt_BR
dc.rightsRestrito*
dc.subjectAnimalsen
dc.subjectAnophelesen
dc.subjectChromosomal Mappingen
dc.subjectChromosome Inversionen
dc.subjectDisease Carrieren
dc.subjectEvolutionen
dc.subjectGeneen
dc.subjectGeneticsen
dc.subjectImmunologyen
dc.subjectPhylogenyen
dc.subjectPolytene Chromosomeen
dc.subjectSouth Americaen
dc.subjectAnimalen
dc.subjectAnophelesen
dc.subjectBiological Evolutionen
dc.subjectChromosome Inversionen
dc.subjectGenes, Insecten
dc.subjectInsect Vectorsen
dc.subjectPhylogenyen
dc.subjectPhysical Chromosome Mappingen
dc.subjectPolytene Chromosomesen
dc.subjectSouth Americaen
dc.titleGNBP domain of Anopheles darlingi: are polymorphic inversions and gene variation related to adaptive evolution?en
dc.typeArtigopt_BR
dc.identifier.doi10.1007/s10709-016-9881-6-
dc.publisher.journalGeneticapt_BR
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