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Title: Genetic diversity and population structure in the Leishmania guyanensis vector Lutzomyia anduzei (Diptera, Psychodidae) from the Brazilian Amazon
Authors: Scarpassa, Vera Margarete
Figueiredo, Adrya da Silva
Alencar, Ronildo Baiatone
Keywords: Microorganism Protein
Dna, Mitochondrial
Coi Gene
Controlled Study
Cytb Gene
Gene Flow
Gene Sequence
Genetic Variability
Habitat Fragmentation
Lutzomyia Anduzei
Population Dispersal
Population Structure
Priority Journal
Species Habitat
Vector Control
Disease Carrier
Gene Frequency
Leishmania Guyanensis
Mitochondrial Gene
Genetics, Population
Leishmania Guyanensis
Gene Frequency
Genes, Mitochondrial
Genetic Variation
Genetics, Population
Insect Vectors
Leishmania Guyanensis
Issue Date: 2015
metadata.dc.publisher.journal: Infection, Genetics and Evolution
metadata.dc.relation.ispartof: Volume 31, Pags. 312-320
Abstract: Lutzomyia (Nyssomyia) anduzei has been recognized as a secondary vector of Leishmania guyanensis in the Brazilian Amazon region. Since L. anduzei is anthropophilic, co-exists in areas of high leishmaniasis transmission and has been found infected with L. guyanensis, the understanding of the vector population structure and of the process responsible for it is paramount to the vector management and control efforts. In this study we analyzed 74 and 67 sequences of the COI and Cytb loci, respectively, from mitochondrial DNA, aiming to estimate the intra-population genetic variability and population structure in six L. anduzei samples from the Brazilian Amazon region. For COI, we found 58 haplotypes, low to high (FST=0.0310-0.4128) and significant (P=0.0033) genetic structure, and reduced gene flow among populations. The haplotype network yielded many reticulations that likely resulted from hypervariability in the locus. For Cytb, we observed 27 haplotypes, low to moderate (FST=0.0077-0.1954) and nonsignificant (P>0.05) genetic structure for the majority of comparisons and extensive gene flow among populations, in line with the haplotypes network data. AMOVA analysis indicated that most of the variation occurred within populations (83.41%, 90.94%); nevertheless, there were significant differences (ΦST=0.0906-0.1659; P=0.00098; P=0.00000) among them for both loci. The Mantel test showed that the genetic structure is not associated to an isolation-by-distance (IBD) model in either of both loci. These data suggest that L. anduzei is genetically very diverse. The genetic structure lacking IBD may be due to adaptation to local habitats and the low dispersal capacity of the sandflies, and both could lead to population fragmentation and geographic isolation. These findings have important implications for epidemiology, surveillance and vector control and may be a first step in understanding the evolutionary history of this species. © 2015 Elsevier B.V.
metadata.dc.identifier.doi: 10.1016/j.meegid.2015.02.007
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