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Campo DC | Valor | Idioma |
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dc.contributor.author | Manfredo Vieira, Silvio | - |
dc.contributor.author | Silva, Rangel Leal | - |
dc.contributor.author | Lemos, Henrique Paula | - |
dc.contributor.author | Amorim, Rodrigo C.N. | - |
dc.contributor.author | Silva, Ellen Cristina Costa | - |
dc.contributor.author | Reinach, Peter Sol | - |
dc.contributor.author | Cunha, Fernando Queiroz | - |
dc.contributor.author | Pohlit, Adrian Martin | - |
dc.contributor.author | Cunha, Thiago Mattar | - |
dc.date.accessioned | 2020-06-15T21:48:47Z | - |
dc.date.available | 2020-06-15T21:48:47Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | https://repositorio.inpa.gov.br/handle/1/17680 | - |
dc.description.abstract | Infusions of Picrolemma sprucei roots, stems and leaves are used in traditional medicine throughout the Amazon region from the Guianas to Brazil and Peru in the treatment of gastritis, intestinal helminths and malaria. As there are no studies describing its mode of action in providing a gastroprotective effect, we determined herein that one of the main constituents found in P. sprucei infusions, the quassinoid isobrucein B (IsoB), reduces some of the pathophysiological effects in a mouse model of non-steroidal anti-inflammatory drug (NSAID)-induced gastritis and provides mechanisms of action. Then, IsoB (1.17 g) was isolated from the roots and stems (6.5 kg) of P. sprucei. Its structure was confirmed by 1D and 2D 1H and 13C NMR, ESI-tof-MS, IR and UV. C57BL/6 strain mice were subcutaneously injected with IsoB (0.5-5 mg kg- 1) or vehicle before oral administration of indomethacin and sacrificed later at different time points. Gastric damage was assessed by measuring lesion length. Leukocyte migration was evaluated based on leukocyte rolling and adhesion using intravital microscopy in the mesenteric microcirculation and tissue MPO activity. Stomach extract cytokine (TNFα, IL-1β and KC/CXCL1) and prostaglandin E2 (PGE2) levels were measured by ELISA and RIA, respectively. IsoB pre-treatment (0.5-5.0 mg kg- 1) significantly reduced the formation of indomethacin-induced stomach lesions in a dose-dependent manner. The decrease in stomach lesions was associated with less observed leukocyte rolling, decreased leukocyte adhesion and less neutrophil infiltration (MPO activity). IsoB (1 mg kg- 1) pre-treatment did not prevent indomethacin-induced decreases in stomach PGE2 levels. However, IL-1β and KC/CXCL1 levels were inhibited by this same IsoB dosage, whereas TNF-α was unchanged. IsoB may be a prototypic compound to provide protective effects against NSAID-induced gastritis and possibly other gastropathies. Moreover, IsoB gastroprotective action may be due to a reduction in IL-1β and KC/CXCL1 production/release and leukocyte rolling, adhesion and migration. © 2014 Elsevier B.V. | en |
dc.language.iso | en | pt_BR |
dc.relation.ispartof | Volume 95, Pags. 8-15 | pt_BR |
dc.rights | Restrito | * |
dc.subject | Cxcl1 Chemokine | en |
dc.subject | Interleukin-1beta | en |
dc.subject | Isobrucein B | en |
dc.subject | Nonsteroid Antiinflammatory Agent | en |
dc.subject | Prostaglandin E2 | en |
dc.subject | Prostaglandin Synthase | en |
dc.subject | Protective Agent | en |
dc.subject | Quassinoid Derivative | en |
dc.subject | Tumor Necrosis Factor-alpha | en |
dc.subject | Unclassified Drug | en |
dc.subject | Cytokine | en |
dc.subject | Indometacin | en |
dc.subject | Isobrucein B | en |
dc.subject | Nonsteroid Antiinflammatory Agent | en |
dc.subject | Plant Extract | en |
dc.subject | Prostaglandin E2 | en |
dc.subject | Quassinoid Derivative | en |
dc.subject | Adult | en |
dc.subject | Animals Experiment | en |
dc.subject | Animals Model | en |
dc.subject | Animals Tissue | en |
dc.subject | Capillary Wall | en |
dc.subject | Carbon Nuclear Magnetic Resonance | en |
dc.subject | Controlled Study | en |
dc.subject | Drug Mechanism | en |
dc.subject | Enzyme-linked Immunosorbent Assay | en |
dc.subject | Gastritis | en |
dc.subject | Leukocyte Adherence | en |
dc.subject | Leukocyte Migration | en |
dc.subject | Leukocyte Rolling | en |
dc.subject | Male | en |
dc.subject | Microcirculation | en |
dc.subject | Mouse | en |
dc.subject | Neutrophil Chemotaxis | en |
dc.subject | Nonhuman | en |
dc.subject | Pathophysiology | en |
dc.subject | Picrolemma sprucei | en |
dc.subject | Plant Root | en |
dc.subject | Plant Stem | en |
dc.subject | Priority Journal | en |
dc.subject | Prostaglandin Synthesis | en |
dc.subject | Protection | en |
dc.subject | Proton Nuclear Magnetic Resonance | en |
dc.subject | Simaroubaceae | en |
dc.subject | Stomach Injury | en |
dc.subject | Stomach Lesion | en |
dc.subject | Animals | en |
dc.subject | C57bl Mouse | en |
dc.subject | Cell Adhesion | en |
dc.subject | Chemical Structure | en |
dc.subject | Chemically Induced | en |
dc.subject | Chemistry | en |
dc.subject | Disease Model | en |
dc.subject | Dose Response | en |
dc.subject | Drug Effects | en |
dc.subject | Gastritis | en |
dc.subject | Isolation And Purification | en |
dc.subject | Leukocyte | en |
dc.subject | Medicinal Plant | en |
dc.subject | Metabolism | en |
dc.subject | Neutrophil | en |
dc.subject | Phytotherapy | en |
dc.subject | Simaroubaceae | en |
dc.subject | Stomach Mucosa | en |
dc.subject | Animal | en |
dc.subject | Anti-inflammatory Agents, Non-steroidal | en |
dc.subject | Cell Adhesion | en |
dc.subject | Cytokines | en |
dc.subject | Dinoprostone | en |
dc.subject | Disease Models, Animals | en |
dc.subject | Dose-response Relationship, Drug | en |
dc.subject | Gastric Mucosa | en |
dc.subject | Gastritis | en |
dc.subject | Indomethacin | en |
dc.subject | Leukocytes | en |
dc.subject | Male | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred C57bl | en |
dc.subject | Molecular Structure | en |
dc.subject | Neutrophils | en |
dc.subject | Phytotherapy | en |
dc.subject | Plant Extracts | en |
dc.subject | Plant Roots | en |
dc.subject | Plant Stems | en |
dc.subject | Plants, Medicinal | en |
dc.subject | Quassins | en |
dc.subject | Simaroubaceae | en |
dc.title | Gastro-protective effects of isobrucein B, a quassinoid isolated from Picrolemma sprucei | en |
dc.type | Artigo | pt_BR |
dc.identifier.doi | 10.1016/j.fitote.2014.02.008 | - |
dc.publisher.journal | Fitoterapia | pt_BR |
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