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Title: Phylogenetic analysis of the GST family in Anopheles (Nyssorhynchus) darlingi
Authors: Azevedo Júnior, Gilson Martins de
Guimarães-Marques, Giselle Moura
Cegatti Bridi, Leticia
Christine Ohse, Ketlen
Vicentini, Renato
Tadei, Wanderli Pedro
Rafael, Míriam Silva
Keywords: Glutathione Transferase
Glutathione Transferase
Confidence Interval
Disease Vector
Genetic Analysis
Genetic Structure
Aedes Aegypti
Anopheles Darlingi
Anopheles Gambiae
Chromosome 3
Computer Model
Controlled Study
Culex Quinquefasciatus
Gene Mapping
Gene Sequence
Genetic Variability
Gst Gene
Neighbor Joining Method
Nucleotide Sequence
Sequence Homology
Amino Acid Sequence
Expressed Sequence Tag
Gene Expression Regulation
Molecular Genetics
Multigene Family
Amino Acid Sequence
Expressed Sequence Tags
Gene Expression Regulation, Enzymologic
Glutathione Transferase
Molecular Sequence Data
Multigene Family
Issue Date: 2014
metadata.dc.publisher.journal: Acta Tropica
metadata.dc.relation.ispartof: Volume 136, Número 1, Pags. 27-31
Abstract: Anopheles darlingi Root, 1926 and Anopheles gambiae (Diptera: Culicidae) are the most important human malaria vectors in South America and Africa, respectively. The two species are estimated to have diverged 100 million years ago. Studies on the phylogenetics and evolution of gene sequences, such as glutathione S-transferase (GST) in disease-transmitting mosquitoes are scarce. The sigma class GST (KC890767) from the transcriptome of An. darlingi captured in the Brazilian Amazon was studied by in silico hybridization, and mapped to chromosome 3 of An. gambiae. The sigma class GST of An. darlingi was used for phylogenetic analyses to understand the GST base composition of the most recent common ancestor between An. darlingi, Anopheles gambiae, Aedes aegypti and Culex quinquefasciatus. The GST (KC890767) of An. darlingi was studied to generate the main divergence branches using a Neighbor-Joining and bootstrapping approaches to confirm confidence levels on the tree nodes that separate the An. darlingi and other mosquito species. The results showed divergence between An. gambiae, Ae. Aegypti, Cx. quinquefasciatus, and Phlebotomus papatasi as outgroup, and the homology relationship between sigma class GST of An. darlingi and GSTS1_1 gene of An. gambiae was valuable for phylogenetic and evolutionary studies. © 2014 Elsevier B.V.
metadata.dc.identifier.doi: 10.1016/j.actatropica.2014.03.027
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