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Title: Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae
Authors: Ramos, Santiago Linorio Ferreyra
Macedo, Jeferson Luis Vasconcelos de
Lopes, Maria Teresa Gomes
Batista, Jacqueline da Silva
Formiga, Kyara M.
Silva, Perla Pimentel da
Saulo-Machado, Antonio C.
Veasey, Elizabeth Ann
Keywords: Microsatellite Dna
Dna, Plant
Primer Dna
Genetic Marker
Genetic Variation
Molecular Analysis
Population Structure
Reproductive Strategy
Species Richness
Wild Population
Dna Sequence
Polymorphism, Genetic
Molecular Genetics
Nucleotide Sequence
Polymerase Chain Reaction
Dna Primers
Dna, Plant
Microsatellite Repeats
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Sequence Analysis, Dna
Issue Date: 2012
metadata.dc.publisher.journal: American Journal of Botany
metadata.dc.relation.ispartof: Volume 99, Número 12, Pags. e508-e510
Abstract: Premise of the study: Microsatellite loci were developed for tucumã of Amazonas (Astrocaryum aculeatum), and cross-species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management. Methods and Results: Fourteen microsatellite loci were isolated from a microsatellite-enriched genomic library and used to characterize two wild populations of tucumã of Amazonas (Manaus and Manicoré cities). The investigated loci displayed high polymorphism for both A. aculeatum populations, with a mean observed heterozygosity of 0.498. Amplification rates ranging from 50% to 93% were found for four Astrocaryum species and two additional species of Arecaceae. Conclusions: The information derived from the microsatellite markers developed here provides significant gains in conserved allelic richness and supports the implementation of several molecular breeding strategies for the Amazonian tucumã. © 2012 Botanical Society of America.
metadata.dc.identifier.doi: 10.3732/ajb.1100607
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