Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/18174
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dc.contributor.authorSilva, M. R. da-
dc.contributor.authorMatoso, D. A.-
dc.contributor.authorLudwig, Luis Augusto M.-
dc.contributor.authorGomes, Eder-
dc.contributor.authorAlmeida, Mara Cristina de-
dc.contributor.authorVicari, Marcelo Ricardo-
dc.contributor.authorArtoni, Roberto Ferreira-
dc.date.accessioned2020-06-15T21:52:18Z-
dc.date.available2020-06-15T21:52:18Z-
dc.date.issued2011-
dc.identifier.urihttps://repositorio.inpa.gov.br/handle/1/18174-
dc.description.abstractThe freshwater silver catfish from the genus Rhamdia belongs to a group of neotropical fish with wide distribution throughout Central and South America. The results obtained in the F1 generation of native specimens of this species bred in captivity using molecular cytogenetic tools revealed a high number of triploid specimens (46.8%) in the samples analyzed. The use of double fluorescent in situ hybridization (double-FISH) with 18S and 5S rDNA probes allowed highlighting the location of ribosomal sites with independent activity. This chromosome marker allowed the identification of triploid nuclei, in which three markers were visualized, in contrast to diploid fish, which only exhibited two fluorescent signals. As triploid specimens exhibit either total or partial gonad sterility, the present study demonstrates the importance of cytogenetic monitoring in restocking programs aimed at genetic conservation. © 2011 Springer Science+Business Media B.V.en
dc.language.isoenpt_BR
dc.relation.ispartofVolume 91, Número 3, Pags. 361-366pt_BR
dc.rightsRestrito*
dc.subjectCaptivityen
dc.subjectChromosomeen
dc.subjectConservation Geneticsen
dc.subjectCytogeneticsen
dc.subjectGenetic Markeren
dc.subjectGeographical Distributionen
dc.subjectHybridizationen
dc.subjectMolecular Analysisen
dc.subjectMonitoring Systemen
dc.subjectNative Speciesen
dc.subjectPloidyen
dc.subjectProbeen
dc.subjectSterilityen
dc.subjectTeleosten
dc.subjectVisualizationen
dc.subjectIguacu Basinen
dc.subjectRhamdiaen
dc.subjectRhamdia Quelenen
dc.titleNatural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazilen
dc.typeArtigopt_BR
dc.identifier.doi10.1007/s10641-011-9794-2-
dc.publisher.journalEnvironmental Biology of Fishespt_BR
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