Use este identificador para citar ou linkar para este item:
https://repositorio.inpa.gov.br/handle/1/18239
Título: | Identification of Neotropical felid faeces using RCP-PCR |
Autor: | Roques, Séverine Adrados, Begoña Chávez, Cuauhtémoc Keller, Claudia Magnusson, William Ernest Palomares, F. Godoy, Jose A. |
Palavras-chave: | Dna, Mitochondrial Animals Central America Chemistry Classification Evaluation Feces Felidae Genetics Methodology Polymerase Chain Reaction South America Species Difference Animal Central America Dna, Mitochondrial Feces Felidae Polymerase Chain Reaction South America Species Specificity Felidae Herpailurus Leopardus Wiedii |
Data do documento: | 2011 |
Revista: | Molecular Ecology Resources |
É parte de: | Volume 11, Número 1, Pags. 171-175 |
Abstract: | Faeces similarity among sympatric felid species has generally hampered their use in distributional, demographic and dietary studies. Here, we present a new and simple approach based on a set of species-specific primers, for the unambiguous identification of faeces from sympatric neotropical felids (i.e. puma, jaguar, jaguarundi and ocelot/ margay). This method, referred to as rapid classificatory protocol-PCR (RCP-PCR), consists of a single-tube multiplex PCR yielding species-specific banding patterns on agarose gel. The method was optimized with samples of known origin (14 blood and 15 fresh faeces) and validated in faecal samples of unknown origin (n = 138), for some of which (n = 40) we also obtained species identification based on mtDNA sequencing. This approach proved reliable and provides high identification success rates from faeces. Its simplicity and cost effectiveness should facilitate its application for routine surveys of presence and abundance of these species. © 2010 Blackwell Publishing Ltd. |
DOI: | 10.1111/j.1755-0998.2010.02878.x |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Não existem arquivos associados a este item.
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.